同型半胱氨酸对钙离子激活状态下内皮细胞eNOS活性及NO含量的影响

研究同型半胱氨酸(HCY)在钙离子导体(A23187)刺激下,对内皮细胞内皮型一氧化氮合酶(eNOS)活性及一氧化氮(NO)含量的影响及机制。收集人脐静脉内皮细胞(HUVEC)进行细胞培养,取第二代细胞分4组:对照组,HCY组,A23187组和HCY A23187组。继续培养24h后,测定细胞培养液中NO、丙二醛(MDA)含量和eNOS、超氧化物歧化酶(SOD)活性。结果显示:1)与对照组相比,HCY组培养液中NO含量降低、eNOS活性减弱、MDA含量升高、SOD活力增强(P<0.05);A23187组NO含量升高、eNOS活性增强、MDA含量降低、SOD活力增强(P<0.05)。2)与A23187组相比,HCY A23187组NO含量降低、eNOS活性减弱、MDA含量升高(P<0.05),HCY不影响A23187激活状态下SOD活力的升高(P>0.05)。结论:HCY在静息及钙离子导体(A23187)激活状态下,使HUVEC中NO含量降低e、NOS活性减弱、MDA含量升高,SOD活力代偿性升高。其机制可能与HCY导致的氧化应激抑制eNOS活性及降低NO的生物利用度有关。

The Effect of Homocyeteine on Endothelial Function in the State of Calcium Ionophore

To study the state of calcium ionophore,the effect of homocyeteine on the activites of eNOS and of contents of NO in HUVEC.HUVECs were collected and cultured to the second passage.Then HUVECs were co-cultured with or without homocyeteine or A23187 for 24 hours.The NO,eNOS,MDA,SOD in culture medium were examined.Compared with control group,the activity of eNOS and the content of NO in medium in homocysteine group was significantly reduced but SOD and MDA was significantly increased(P<0.05),In A23187 group,the activity of eNOS and the content of NO was increased significantly,MDA was decreased,SOD was increased.Compared with A23187 group,eNOS and NO was significantly reduced,MDA were increased in HCY A23187 group,but SOD has no difference.Conclusion:homocysteine can decrease the content of NO and the activityof eNOS and increase the content of MDA and SOD in both base and activated state.The mechanisms may be concerned with oxidation stress that can inhibit the activity of eNOS and lower the NO bioavailability.