结合高脂饲料建立酒精性肝损伤大鼠模型的研究

目的研究酒精性肝损伤大鼠模型建立的理想方式。方法取健康雄性SD大鼠30只,随机分为对照组、模型组各15只,模型组给予高脂饲料并灌服白酒,持续四周。实验结束时从大鼠眼眶静脉丛采血,测定血清中谷氨酸氨基转移酶(ALT)、天门冬氨酸转移酶(AST)、总胆固醇(TC)、甘油三脂(TG)、高密度脂蛋白(HDL-C)、肝脏中超氧化物岐化酶(SOD)、丙二醛(MDA)、肝内糖原含量、肝细胞凋亡情况、肝脏系数。结果模型组动物的肝脏指数、血清中的TC、TG和HDL-C、肝糖原、肝脏中MDA的含量、SOD的含量与对照组比较,显著性升高(P<0.05);模型组动物血清中的ALT和AST与对照组比较,显著性升高(P<0.01);模型组动物肝脏的脂肪化、炎症和坏死的评分均明显高于对照组(P<0.05)。结论灌服酒精加以饲喂高脂饲料能成功建立酒精性肝损伤大鼠模型。

Alcohol-induced Liver Injury Combined with High Fat Diet in Rats

Objective To establish an ideal method to develop alcohol-induced liver injury in rats.Method Thirty healthy male Sprague-Dawley(SD) rats were randomly divided into two groups,control group(give NS) and model group(drinking red Star falls and feeding high fat diet),and 15 per group.All the rats were kept on the treatments for 4 weeks.Blood was collected from the orbital venous plexus at the end of experiment,and serum levels of ALT,AST,TC,TG,HDL-C,SOD,MDA,and hepatic glycogen content,as well as liver coefficient were detected. Results The liver coefficient and serum levels of TC,TG and HDL-C,hepatic glycogen,MDA,and SOD in model group increased significantly than those in control(P<0.05);Serum levels of ALT and AST in model group also increased significantly than those in control(P<0.01).Conclusion It is a successful method to develop the alcohol-induced liver injury in rats combining alcohol-drinking with high-fat diet feeding.