水稻条叶枯病毒云南分离物（RStV.Y）的纯化与理化特性分析

采用改进的水稻病毒纯化方法，以２００ｇ／Ｌ的蔗糖垫替代蔗糖密度梯度，从ＲＳｔＶ感染的水稻叶片中制备了病毒颗粒，电镜观察显示，ＲＳｔＶ为细丝状和分枝状结构，变性琼脂糖凝胶电泳分析分离纯化的病毒总ＲＮＡ得到４个大小不 同的病毒ＲＮＡ。

Purification,Physical and Chemical Characterization of Yunnan Isolate of Rice Stripe Virus

Rice stripe virus (RStV) from infected rice leaf was purified by a modified procedure of conventional purification with 200 g/L sucrose cushion instead of linear sucrose gradient. The virus with filamentous and branched filamentous particles were observed in purified preparations under electron microscope. Significant size differences were found in the single stranded forms of genomic RNAs of RStV by denatured agarose gel electrophoretic analysis. The viral coat protein was detected in SDS PAGE. With the antibody of RStV form Japan,the virus showed a serological homology. Thus,it indicates that this simple protocol is suitable for purifying the RNP particles of RStV.