| 两种细胞色素b5水溶性结构域的性质结构比较 |
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| 引用本文： | 孙雨龙,黄燕.两种细胞色素b5水溶性结构域的性质结构比较[J].复旦学报(自然科学版),1999,38(6):611-616. |
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| 作者姓名： | 孙雨龙 黄燕 |
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| 作者单位： | 复旦大学化学系!上海200433 |
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| 基金项目： | 国家自然科学基金!29731030 |
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| 摘 要： | 分别将编码牛肝微粒体细胞色素ｂ５水溶性结构域片段Ａ１ａ７－Ａｒｇ８８和Ｓｅｒ５－Ｓｅｒ９７基因在大肠杆菌ＪＭ８３中高效表达，分离纯化后得到Ｔｂ５和Ｌｂ５２种重组野生型蛋白。用核磁共振、紫外可见光谱、圆二色散光谱、循环伏安等方法研究结果表明，Ｌｂ５肽链２端残基未能使蛋白质的血红素周围环境与Ｔｂ５蛋白质Ｎ末端的Ｓｅｒ５和Ｌｙｓ６２个残基所贡献。
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| 关 键 词： | 细胞色素b5 结构 氧化还原蛋白 水溶性结构构域 |
Comparison of Property and Structure between Two Kinds of Water-Soluble Domains of Cytochrome b_5 |
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| SUN Yu-long,WANG Wen-hu,HUANG Yan,WANG Yun-hua,HUANG Zhong-xian.Comparison of Property and Structure between Two Kinds of Water-Soluble Domains of Cytochrome b_5[J].Journal of Fudan University(Natural Science),1999,38(6):611-616. |
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| Authors: | SUN Yu-long WANG Wen-hu HUANG Yan WANG Yun-hua HUANG Zhong-xian |
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| Institution: | SUN Yu-long;WANG Wen-hu;HUANG Yan;WANG Yun-hua;HUANG Zhong-xian(1. Department of Chemistry)XIE Yi;WU Xiao-zhou(2. Institute of Genetics) |
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| Abstract: | Bovine liver microsomal gene encoding water-soluble heme-containing fragment of cytochrome b5 was expressed in E. coli JM83. Two recombinate wild type cytochrome Lb5 and Tb, were isolated and purified.Studies by NMR,UV-visible,Circular dichroism(CD),cyclic voltammetry (CV) etc. show that the increase of the terminal residues on Lb, did not cause significant difference of the heme pocket from the Tb5. But the study on the protein stabillty to denaturant and heat shows that the Cyt Lb, structural stability is more stable than Cyt Tb5,that can be attributed to the N-terminal residues Ser5 and Lys6 of Cyt Lb5. |
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| Keywords: | cytochrome b_5 effect of terminal-residues structure property |
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