| p21基因在小鼠胚胎发育过程中的表达 |
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| 引用本文： | 李鹂,黄衡宇,谢永芳.p21基因在小鼠胚胎发育过程中的表达[J].吉首大学学报(自然科学版),2006,27(2):80-83. |
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| 作者姓名： | 李鹂 黄衡宇 谢永芳 |
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| 作者单位： | （1.吉首大学生物资源与环境科学学院，湖南 吉首 416000；2.云南大学生命科学学院，云南 昆明 650091；3.重庆邮电学院生物信息学院，重庆 400065） |
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| 摘 要： | 以E9日龄至E14日龄昆明种正常小鼠胚胎为材料，利用质粒扩增的、地高辛标记的基因探针在组织切片上进行DNA-mRNA分子原位杂交，研究了p21基因在小鼠胚胎发育过程中的表达.结果表明:p21基因从E10日开始参与小鼠胚胎发育，其表达特异性随着胚胎发育进程逐渐增强，与它在细胞周期中的负调控作用相一致.p21基因表达强度较稳定，与其mRNA稳定有关.
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| 关 键 词： | 原位杂交 p21基因 细胞周期调控 基因表达 |
| 文章编号： | 1007-2985（2006）02-0080-04 |
| 收稿时间： | 2006-02-09 |
| 修稿时间： | 2006年2月9日 |
Study of Gene Expression of p21 with in Situ Hybridization Technique |
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| LI Li,HUANG Heng-yu,XIE Yong-fang.Study of Gene Expression of p21 with in Situ Hybridization Technique[J].Journal of Jishou University(Natural Science Edition),2006,27(2):80-83. |
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| Authors: | LI Li HUANG Heng-yu XIE Yong-fang |
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| Institution: | （1.College of Resources and Environmental Science,Jishou University，Jishou 416000，Hunan China;2.the Biology Department of College of Life Science and Chemistry;Yunnan University，Kunming 650091，Yunnan China;3.College of Bioformatic,Chongqing of Post and Telecommunications University,Chongqing 400065,China) |
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| Abstract: | Using in situ hybridization technique with dig labeled probe,the expression of p21 gene in mice's embryo development has been studied in this paper.The results can be summarized as follows:p21 gene does not take part in the formation of organ primordial during the development of embryo of age 9ds.It starts to express in the embryo of age 10ds and then express stronger with the development of the embryo.It seems to suggest that p21 gene is concord with the negative regulation of cell cycle.The little change in expression strengthen may relates with its stable mRNA. |
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| Keywords: | in situ hybridization p21 cell cycle regulation gene expression |
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