| 淀粉聚丙烯酰胺凝胶电泳鉴定耐热糖化酶组分 |
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| 引用本文： | 武金霞,张贺迎. 淀粉聚丙烯酰胺凝胶电泳鉴定耐热糖化酶组分[J]. 河北大学学报(自然科学版), 2004, 24(4): 415-418. DOI: 10.3969/j.issn.1000-1565.2004.04.018 |
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| 作者姓名： | 武金霞 张贺迎 |
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| 作者单位： | 河北大学,生命科学学院,河北,保定,071002 |
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| 摘 要： | 采用微波诱变筛选出一突变株,该突变株粗酶液的最适作用温度比出发株的高20℃;建立了一种Starch-PAGE检测粗酶液中具有糖化酶活性组分的方法,测定出突变株含有4个糖化酶组分;并建立了SDS-Starch-PAGE检测耐热糖化酶组分的方法,鉴定出突变株含有3个耐热糖化酶组分.
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| 关 键 词： | 糖化酶 耐热性 SDS-Starch-PAGE |
| 文章编号： | 1000-1565(2004)04-0415-04 |
| 修稿时间： | 2003-09-03 |
Identification of Thermostable Glucoamylase Components by Starch-PAGE |
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| WU Jin-xia,ZHANG He-ying. Identification of Thermostable Glucoamylase Components by Starch-PAGE[J]. Journal of Hebei University (Natural Science Edition), 2004, 24(4): 415-418. DOI: 10.3969/j.issn.1000-1565.2004.04.018 |
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| Authors: | WU Jin-xia ZHANG He-ying |
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| Abstract: | One mutant which produces thermostable glucoamylase was obtained through microwave mutation, the optimum temperature of the crude enzyme from the mutant was 20 ℃ higher than that of primitive strain; A method of Starch-PAGE was established by which the components having glucoamylase activity can be detected and four active components of crude enzyme were detected; A convenient method of SDS-Starch-PAGE was also established by which thermostable glucoamylase components can be detected easily, and three thermostable glucoamylase components were detected. |
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| Keywords: | glucoamylase thermostable SDS-starch-PAGE |
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