| 固定化脂肪酶催化小桐子毛油合成生物柴油 |
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| 引用本文： | 李俊奎,王芳,谭天伟,邓利. 固定化脂肪酶催化小桐子毛油合成生物柴油[J]. 北京化工大学学报(自然科学版), 2010, 37(2): 100-103. DOI: 10.3969/j.issn.1671-4628.2010.02.020 |
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| 作者姓名： | 李俊奎 王芳 谭天伟 邓利 |
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| 作者单位： | 北京化工大学 北京市生物加工过程重点实验室， 北京 100029 |
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| 基金项目： | 国家自然科学基金，国家"863"计划，国家"973"计划 |
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| 摘 要： | 研究了以石油醚提取的小桐子毛油和甲醇为原料,利用固定化Candidasp.99-125脂肪酶催化反应合成生物柴油的可行性。考察了几种因素对酶法催化合成脂肪酸甲酯的影响。结果表明以小桐子毛油的质量为基准,在水含量为10%,溶剂量为2mL/g,脂肪酶量为20%,温度为40℃的条件下,3次流加甲醇,12 h后单批最高产率可达93%。在此条件下固定化酶连续使用14批,产率仍然可保持在70%以上。
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| 关 键 词： | 生物柴油 小桐子毛油 固定化酶 |
| 收稿时间： | 2009-04-08 |
Synthesis of biodiesel from crude jatropha oil using immobilized lipase |
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| LI JunKui,WANG Fang,TAN TianWei,DENG Li. Synthesis of biodiesel from crude jatropha oil using immobilized lipase[J]. Journal of Beijing University of Chemical Technology, 2010, 37(2): 100-103. DOI: 10.3969/j.issn.1671-4628.2010.02.020 |
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| Authors: | LI JunKui WANG Fang TAN TianWei DENG Li |
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| Affiliation: | Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, Beijing 100029, China |
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| Abstract: | The enzymatic production of fatty acid methyl esters(FAMEs)from crude jatropha oil and methanol u-sing the immobilized lipase Candida sp.99-125 has been studied.The crude oil was extracted by a simple meth-od.The efiect of varying water content,solvent content,enzyme amount,temperature and number of additions of methanol on the reaction were investigated.The optimal conditions for processing 1 g of crude jatropha oil were found to be:10% water,2 mL petroleum ether as solvent,20%lipase based on the weight of oil,temperature 40 ℃,and three-step addition of methano1.The maximum yield of FAMEs was 93%after 12h and after 14 cycles the yield of FAMEs was still above 70%. |
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| Keywords: | biodiesel crude jatropha oil immobilized lipase |
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