中药黄芩提取方法的优化

运用均匀设计法优化中药黄芩提取工艺,以HPLC法测定黄芩中黄芩苷的含量,并以此为指标考察乙醇体积分数、乙醇溶剂用量、浸泡时间、蒸馏时间和黄芩粒度5个因素对黄芩苷提取收率的影响.实验确定乙醇体积分数为60%,乙醇溶剂用量为70~90 mL,浸泡时间为0~3 h,回流时间为120 min,黄芩粒度取0.1~0.5 mm为最佳的提取条件.本工艺设计合理,方法方便实用,预测性强,是优化中草药提取工艺的一个非常有效的工具.

Optimization of Experimental Parameters for Extracting Baicalin from Scutellaria baicalensis Georgi

To optimize all experimental parameters for extracting Baicalin from Scutellaria baicalensis Georgi,five factors such as alcohol concentration,volume of solvent,the time of immersion and extraction,as well as the herb granularity were selected and 10 sets of extraction experiments were designed according to the U-(10)(10~5) uniform design form.For each extract,no peak was observed to disturb the baicalin in chromatogram.The concentration of baicalin in each extraction experiment was calculated based on the calibration curve constructed with a series of baicalin standards with different concentrations.The optimized extraction parameters were determined by the multiple regression analysis.Experimental results showed that it could get much more yield of baicalin if 60% of alcohol concentration,70～90 mL of alcohol,0～3 h of dip time,120 min of extraction time and 0.1～0.5 mm of granularity were selected,and more stable multiple regression result could be obtained if much more experimental sets would be arranged.