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| 高温大曲中筛选产纤维素酶的耐高温芽孢杆菌 | |
| 引用本文： | 龚丽琼,邓朝霞,黄祖新,颜林春,马校卫,汤二将,陈由强,陈如凯. 高温大曲中筛选产纤维素酶的耐高温芽孢杆菌[J]. 福建师范大学学报(自然科学版), 2012, 28(3): 106-112 |
| 作者姓名： | 龚丽琼 邓朝霞 黄祖新 颜林春 马校卫 汤二将 陈由强 陈如凯 |
| 作者单位： | 1. 福建师范大学生命科学学院,福建福州350108;农业部甘蔗生理生态与遗传改良重点开放实验室,福建福州350108;福建省发育与神经生物学重点实验室,福建福州350108 2. 福建师范大学生命科学学院,福建福州350108;农业部甘蔗生理生态与遗传改良重点开放实验室,福建福州350108;福建省发育与神经生物学重点实验室,福建福州350108;教育部工业微生物工程研究中心,福建福州350108 3. 农业部甘蔗生理生态与遗传改良重点开放实验室,福建福州,350108 |
| 基金项目： | 国家现代农业产业技术体系建设专项资金资助项目，福建省教育厅资助项目 |
| 摘 要： | 从高温大曲中筛选出产纤维素酶的芽孢杆菌1株,进行形态特征、生化鉴定和16S rDNA序列分子鉴定,该菌株鉴定为Bacillus cereusFrankland.对该菌株进行产酶摇瓶液体发酵,测定其内切纤维素酶活力和外切纤维素酶活力分别为260.32,87.22u.mL-1,对该菌株进行产酶固态发酵,测定其内切纤维素酶活力和外切纤维素酶活力分别为556.64,121.47u.mL-1.酶的稳定性实验显示该菌株产纤维素酶在60℃以下和在pH4～8范围内具有良好的热稳定性和pH稳定性,利用响应面法优化固态发酵产酶培养基,优化后测定纤维素酶活提高至1 643.27u.mL-1. |
| 关 键 词： | 大曲 纤维素酶 芽胞杆菌 液体发酵 固态发酵 响应面优化 |
Screening of a Thermostable Cellulase-secreting Bacillus from High Temperature Daqu |
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| GONG Li-qiong , DENG Zhao-xia , HUANG Zu-xin , YAN Lin-chun , MA Xiao-wei , TANG Er-jiang , CHEN You-qiang , CHEN Ru-kai. Screening of a Thermostable Cellulase-secreting Bacillus from High Temperature Daqu[J]. Journal of Fujian Teachers University(Natural Science), 2012, 28(3): 106-112 | |
| Authors: | GONG Li-qiong DENG Zhao-xia HUANG Zu-xin YAN Lin-chun MA Xiao-wei TANG Er-jiang CHEN You-qiang CHEN Ru-kai |
| Affiliation: | 1.College of Life Sciences,Fujian Normal University,Fuzhou 350108,China;2.Key Laboratory of Sugarcane Genetics and Breeding,Ministry of Agriculture,Fuzhou 350108,China;3.Fujian Key Laboratory of Developmental and Neurobiology,Fuzhou 350108,China;4.Engineering Research Center of Industrial Microbiology,Ministry of Education,Fuzhou 350108,China) |
| Abstract: | The Bacillus that can produce cellulase is isolated and purified from the high temperature Daqu,based on the analysis of 16SrDNA and some biochemical and physiological characteristics,this strain was identified as Bacillus cereus Frankland.The shake flask liquid fermentation was taken to determine the endo-cellulase activity(260.32 u·mL-1),and exo-cellulase activity(87.22 u·mL-1),the solid state fermentation was taken to determine the endo-cellulase activity(556.64 u·mL-1)and exo-cellulase activity(121.47 u·mL-1).High heat and pH stability of cellulase activity are observed at temperature lower than 60 ℃ and pH4～8.After the response surface method optimization,the cellulase activity of solid state fermentation increased to 1 643.27 u·mL-1. |
| Keywords: | Daqu cellulase Bacillus liquid fermentation solid state fermentation response surface methodology |
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