| Pichia酵母宿主细胞DNA残留量检测方法的建立 |
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| 引用本文： | 刘晓志,高健,赵伟,刘艳玲,王志明,贾茜. Pichia酵母宿主细胞DNA残留量检测方法的建立[J]. 河北师范大学学报(自然科学版), 2012, 36(1): 80-83 |
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| 作者姓名： | 刘晓志 高健 赵伟 刘艳玲 王志明 贾茜 |
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| 作者单位： | 华北制药集团新药研究开发有限责任公司,河北石家庄050015;抗体药物研制国家重点实验室,河北石家庄050015 |
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| 基金项目： | 国家"十一五"重大新药创制项目 |
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| 摘 要： | 为检测重组人血白蛋白中外源性DNA残留量,以宿主酵母工程菌基因组DNA为模板,使用地高辛标记探针进行点杂交.结果证明:该方法检测灵敏度较好,特异性较强,操作安全简便,可用于重组人血白蛋白的检测.
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| 关 键 词： | 地高辛 探针 DNA残留量 重组人血白蛋白 |
Development of a Method for Determination of Residual DNA in Pichia Pastoris Host Cells |
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| LIU Xiaozhi , GAO Jian , ZHAO Wei , LIU Yanling , WANG Zhiming , JIA Qian. Development of a Method for Determination of Residual DNA in Pichia Pastoris Host Cells[J]. Journal of Hebei Normal University, 2012, 36(1): 80-83 |
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| Authors: | LIU Xiaozhi GAO Jian ZHAO Wei LIU Yanling WANG Zhiming JIA Qian |
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| Affiliation: | 1,2(1.New Drug Research and Development Company Limited,North China Pharmaceutical Corporation,Hebei Shijiazhuang 050015,China;2.State Key Laboratory of Antibody Drug Development,Hebei Shijiazhuang 050015,China) |
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| Abstract: | To detect the residues DNA in recombinant human serum albumin,dot blot is done by using pichia pastovis host cells DNA of tempate and using Digoxin as probe.Results show that the method is of high sensitivity,strong specificity,safe and easy operation,it can be used for verification the recombinant human serum albumin. |
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| Keywords: | Digoxin probe residues DNA recombinant human serum albumin |
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