| 花椰菜天然钙调素抗体的特性及其ELISA定量法的建立 |
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| 引用本文： | 郑海金 张利平. 花椰菜天然钙调素抗体的特性及其ELISA定量法的建立[J]. 兰州大学学报(自然科学版), 1995, 31(2): 116-121 |
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| 作者姓名： | 郑海金 张利平 |
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| 作者单位： | 兰州大学生物学系，中科院兰州沙漠所 |
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| 基金项目： | 甘肃省自然科学基金，中科院沙漠所沙坡头实验站资助 |
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| 摘 要： | 钙调素是一种具有多种调节功能的钙结合蛋白质，其免疫原性很弱，本文采用两次基础免疫和多次加强免疫的方法，获得了免疫花椰菜天然钙调素抗血清，用免疫双扩散法鉴定，其效价为１：３２钙调节不易与固相载体结合，我们先用０．２％戊二醛处理聚苯乙烯板载体，再用于包被钙调素，在上述基础上建立了定量测定植物钙调素的竞争性酶联免疫吸附测定（ＥＬＩＳＡ）技术及检测动物血清中抗钙调素抗体的间接ＥＬＩＳＡ技术。
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| 关 键 词： | 酶联免疫吸附 测定 免疫原性 花椰菜 调钙蛋白 |
Characterization of Antibody against Plant Natural Calmodulin and Establishment of Quantitative ELISA |
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| Zheng Haijin,Hu Jiancheng,Chen Xinyang,Wang Wei,Li Weiming,Wen Xiao,Zhou Guangming,Zhu Weihua. Characterization of Antibody against Plant Natural Calmodulin and Establishment of Quantitative ELISA[J]. Journal of Lanzhou University(Natural Science), 1995, 31(2): 116-121 |
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| Authors: | Zheng Haijin Hu Jiancheng Chen Xinyang Wang Wei Li Weiming Wen Xiao Zhou Guangming Zhu Weihua |
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| Abstract: | Calmodulin(CaM)is a Ca2+ -combined protein with multiple regulating functions.and with weak immunogenicity. We got the rabbit serum against cauliflower natural CaM through two basic and multiple enhancing immunizations, its titer was 1:32 by immune double diffusion determination. CaM was not well combined with solid carrier, so we treated polyphenyl ethylene plate with 0. 2% glutaraldehyde before the plate was packed. The technique of competitive enzyme-linked immunoabsorbance assay (ELISA) for quantitatively determining plant CaM and indirect ELISA for detecting antibody against self-CaM in sera of patients of some diseases was set up. |
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| Keywords: | calmodulin immunogenicity ELISA immune double diffusion titer |
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