The pathogenicity on legumes of Cucumber mosaic virus was determined by 243 nucleotides on 2a polymerase gene of viral RNA2 |
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Authors: | Xiaorong Tao Xueping Zhou Guixin Li Jialin Yu |
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Institution: | (1) Institute of Biotechnology, Zhejiang University, 310029 Hangzhou, China;(2) National Key Laboratory on Agrobiotechnology, China Agriculture University, 100094 Beijing, China |
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Abstract: | We had isolated and identified two Cucumber mosaic virus (CMV) isolates, the CMV red bean (CMV-RB) isolate and the CMV pea (CMV-P1) isolate. CMV-RB induces necrotic local lesions
on inoculated leaves of broad bean, pea, cowpea and bean, and could not infect these hosts systemically. However, CMV-P1 was
able to infect these legumes systemically. To study the difference of pathogenicity on the legumes induced by these two CMV
isolates, the full-length infectious cDNA clones of CMV-Fny, which induced similar symptoms as CMV-RB in the four legumes,
were used. The 243 nucleotides fragment, which encodes highly conserved GDD amino acid motif on 2a replicase gene of CMV-Fny
RNA2, was replaced with that of CMV-P1. The constructed chimeric virus FP could infect these legumes systemically. The exchange
of this region changes the virus symptoms on the legumes, indicating that this 243 nucleotides fragment has major effect on
pathogenicity of CMV on the legumes. |
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Keywords: | Cucumber mosaic virus legumes replicase gene pathogenicity |
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