| 基于包埋在DNA-碳纳米管复合物中的HRP修饰电极的过氧化氢传感器的研制 |
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| 引用本文： | 严楠,宋永海,汪莉,崔康.基于包埋在DNA-碳纳米管复合物中的HRP修饰电极的过氧化氢传感器的研制[J].江西师范大学学报(自然科学版),2009,33(3). |
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| 作者姓名： | 严楠 宋永海 汪莉 崔康 |
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| 作者单位： | 江西师范大学,化学化工学院,江西,南昌,330022 |
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| 基金项目： | 国家自然科学基金，江西省教育厅科技项目，江西师范大学科研启动基金，江西师范大学大学生学术课题 |
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| 摘 要： | 将辣根过氧化物酶(HRP)包埋于DNA-碳纳米管复合物中后再修饰到玻碳电极上制得过氧化氢传感器.DNA和碳纳米管的复合物为酶分子提供了一个生物兼容性很好的微环境,使HRP在电极上的生物活性大大增强.此修饰电极对过氧化氢的还原表现出了很好的催化能力,同时我们还对其它因素影响进行了研究.实验表明,此传感器能在很短的时间(5 s)内对过氧化氢有很好的响应.其检测线为2.0 mol/L,检测范围为10.0μmol/L～10.2mmol/L.
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| 关 键 词： | 辣根过氧化酶(HRP) 碳纳米管(CNTS) 玻碳电极 GCE.生物传感器 |
A New Biosensor Based on Horseradish Peroxidase Immobilized on SCNTs and DNA Complex Composite Film on a Carbon Electrode |
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| YAN Nan,SONG Yong-hai,WANG Li,CUI Kang.A New Biosensor Based on Horseradish Peroxidase Immobilized on SCNTs and DNA Complex Composite Film on a Carbon Electrode[J].Journal of Jiangxi Normal University (Natural Sciences Edition),2009,33(3). |
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| Authors: | YAN Nan SONG Yong-hai WANG Li CUI Kang |
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| Institution: | Institute of Chemistry and Chemical Enginerring;Jiangxi Normal University;Nangchang 330022;China |
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| Abstract: | A novel method to fabricate a hydrogen peroxide sensor was developed by immobilizing horseradish peroxidase(HRP) on a carbon nanotubes(CNTs)-DNA compatible film which were directly dropped on glass carbon electron(GCE).The DNA-CNTs composite film obtained provided a biocompatible microenvironment for enzyme molecules and a tunnel to transfer electron.The immobilized HRP displayed good electrocatalytic response to the reduction of hydrogen peroxide.The performance and factors influencing the resulted sensor ... |
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| Keywords: | DNA |
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