| 人源fidgetin like-1蛋白AAA结构域的重组表达及酶学性质分析 |
| |
| 引用本文： | 彭雯韬,王春光. 人源fidgetin like-1蛋白AAA结构域的重组表达及酶学性质分析[J]. 上海师范大学学报(自然科学版), 2011, 40(5): 533-539 |
| |
| 作者姓名： | 彭雯韬 王春光 |
| |
| 作者单位： | 同济大学生命科学与技术学院,上海,200092 |
| |
| 基金项目： | 国家自然科学基金项目(31070690) |
| |
| 摘 要： | Fidgetin是2000年发现的一个AAA蛋白家族新成员,同源序列分析表明它与ka-tanin、spastin属于AAA家族的同一亚家族,因此可能也具有ATP依赖的微管切割活性,ATP的结合和水解应该发生于fidgetin蛋白中保守的AAA结构域.在大肠杆菌中重组表达的人源fidgetin like-1(FIGL-1)蛋白的AAA结构域片段(HsFIGL-1-AAA),纯化后的最终产率为每升5 mg蛋白.与AAA蛋白通常形成六聚体不同,HsFIGL-1-AAA在溶液中为单体,且其ATPase活性很低,仅为0.0063 s-1.与其他AAA蛋白的序列比对发现,Sensor 2 motif中保守的Arg残基在HsFIGL-1-AAA结构域中为Thr,但该位点的突变T609R并未明显改变该蛋白的ATPase活性.这些结果提示HsFIGL-1可能以一种特殊的方式发挥功能,这为进一步研究fidgetin及其同源蛋白的结构和功能奠定了基础.
|
| 关 键 词： | fidgetin AAA蛋白 ATPase |
Recombinant expression and activity analysis of the AAA domain of human fidgetin like-1 |
| |
| PENG Wen-tao,WANG Chun-guang. Recombinant expression and activity analysis of the AAA domain of human fidgetin like-1[J]. Journal of Shanghai Normal University(Natural Sciences), 2011, 40(5): 533-539 |
| |
| Authors: | PENG Wen-tao WANG Chun-guang |
| |
| Affiliation: | PENG Wen-tao,WANG Chun-guang(School of Life Sciences and Technology,Tongji University,Shanghai 200092,China) |
| |
| Abstract: | Fidgetin is a new AAA protein cloned in 2000,because it has an AAA domain at its C-terminus.Together with two microtubule-severing proteins katanin and spastin,fidgetin is classified into subfamily-7 of AAA family,which implies that fidgetin may have ATP-dependent microtubule-severing activity and that the conserved AAA domain is responded for the ATP binding and hydrolysis.In order to understand the activity of its AAA domain,the AAA domain of human fidgetin like-1(HsFIGL-1-AAA) was expressed in E.coli wit... |
| |
| Keywords: | fidgetin AAA protein family ATPase |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
