| 遗传定位一个花粉发育相关的拟南芥新基因SPG1 |
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| 引用本文： | 韦嘉励, 周树敏, 袁晓君, 张卫.遗传定位一个花粉发育相关的拟南芥新基因SPG1[J].上海师范大学学报(自然科学版),2011,40(5):522-527. |
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| 作者姓名： | 韦嘉励 周树敏 袁晓君 张卫 |
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| 作者单位： | 上海大学生命科学学院,上海,200444 |
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| 基金项目： | The Excellent Young Teacher Foundation of Shanghai under Grant(shu08093); National Natural Science Foundation of China(30870225); Technical Innovation Fund of Shanghai University; Key Project of Shanghai Committee of Science and Technology |
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| 摘 要： | 通过EMS诱变获得一个拟南芥突变体shrunken pollen grain1,spg1.根据横切片的观察结果,与野生型相比较,突变体观察到异常的花粉粒.遗传学的实验表明:spg1突变现象由一个隐性的单基因控制.通过图位克隆的方法,spg1突变位点定位在3号染色体的分子标记3-AL138638-6632和3-AL353865-6814之间大约427kb的区间内.生物信息学的分析表明,在这个区间内没有任何已知的与育性相关的基因.以上结果说明SPG1是一个控制拟南芥雄性不育的新基因.
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| 关 键 词： | 拟南芥 花药 雄性不育 小孢子 图位克隆 |
Genetic mapping of a new Arabidopsis gene SPG1 responsible for microspore development |
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| WEI Jia-li,ZHOU Shu-min,YUAN Xiao-jun,ZHANG Wei.Genetic mapping of a new Arabidopsis gene SPG1 responsible for microspore development[J].Journal of Shanghai Normal University(Natural Sciences),2011,40(5):522-527. |
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| Authors: | WEI Jia-li ZHOU Shu-min YUAN Xiao-jun ZHANG Wei |
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| Institution: | WEI Jia-li,ZHOU Shu-min,YUAN Xiao-jun,ZHANG Wei(School of Life Sciences,Shanghai University,Shanghai 20444,China) |
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| Abstract: | An Arabidopsis male sterile mutantshrunken pollen grain1,spg1 was obtained through enthyl methane sulfona(EMS) mutagenesis strategy.By the observation of the transverse section,the mutant showed the aberrant microspores and pollen grains compared with wild type.Genetic assay indicated thatspg1 phenotype was controlled by a single recessive mutant locus,and this locus was mapped to the region about 427kb flanked with genetic marker 3-AL138638-6632 and 3-AL353865-6814 on chromosome 3 by map-based cloning appr... |
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| Keywords: | Arabidopsis anther male sterile microspore map-based cloning |
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