| 枯草芽孢杆菌D-核糖摇瓶发酵条件的研究 |
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| 引用本文： | 杨柳,张克旭,刘淑云,陈宁.枯草芽孢杆菌D-核糖摇瓶发酵条件的研究[J].天津科技大学学报,2001(1):11-13. |
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| 作者姓名： | 杨柳 张克旭 刘淑云 陈宁 |
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| 作者单位： | 天津轻工业学院食品工程系, |
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| 摘 要： | 对枯草芽孢杆菌(Bacillus subtilis)S21进行了摇瓶条件下的D-核糖发酵条件的研究，主要考察了添加木糖、pH、溶氧水平以及采用补糖方式对发酵的影响，确定了D-核糖发酵的最佳工艺条件D-木糖50g／L初始pH7．0，初始葡萄糖浓度为150g／L，碳酸钙20g／L，并在发酵36h后，在装液量为35mL的500mL三角瓶中添加0．75g／mL的葡萄糖溶液2mL，S21菌可积累D-核糖达51．1g／L。
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| 关 键 词： | D-核糖 摇瓶发酵 补料培养 |
| 文章编号： | 1001-456X（2001）01-0011-03 |
| 修稿时间： | 2000年4月24日 |
STUDIES ON THE CONDITIONS OF D-RIBOSE FERMENTATION BYBACILLUS SUBTILIS MUTANT S21 |
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| YANG Liu,ZHANG Ke-xu,LIU Shu-yun,CHEN Ning.STUDIES ON THE CONDITIONS OF D-RIBOSE FERMENTATION BYBACILLUS SUBTILIS MUTANT S21[J].Journal of Tianjin University of Science & Technology,2001(1):11-13. |
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| Authors: | YANG Liu ZHANG Ke-xu LIU Shu-yun CHEN Ning |
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| Abstract: | Shake-flask culture conditions of D-ribose fermentation were studied with a Bacillus subtilis mutant S21.The paper investigated the factors affecting the yield of D-ribose,such as the amount of D-xylose in fermentation medium,pH、dissolved oxygen concentration and sugaring culture.It was suggested that the better fermentation conditon on laboratory scale was as following:D-xylose 50 g／L,initial pH7.0,initial glucose concentration 150 g／L,CaCO320 g／L．After it cultured 36 h,2 mL 0．75 g／mL D-glucose solution should be added to the 500 mL flask contained 35 mL fermentation medium.Under such optimized conditions,the yield of D-ribose was about 51.1 g／L． |
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| Keywords: | D-ribose fermentation feed-batch culture |
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