Maize transformation via pollen tube pathway and the inheritance of transgene in progeny

The herbicide-resistant gene als was introduced into maize inbred line Qi319 through pollen-tube pathway. The inheritance of transgene was studied up to 33 generation. Transformation was performed by applying plasmid DNA carrying solution on the severed styles after self-pollination, or just prior to selfpoll/nation. After herbicide screening, PCR and Southern blot analysis, seventeen plants were confirmed positive in TO generation, and sixteen of them were self-bred to construct family to 33 progeny. Through PCR analysis and herbicide screening in offspring, four lines were confirmed following a 3 : 1 Mendelian segregation ratio. In other lines the numbers of positive plants were lower than expected. Therefore, it is necessary to select large population in progeny to produce stable inherited lines when transgene was delivered by pollen tube method.

Maize transformation via pollen tube pathway and the inheritance of transgene in progeny

The herbicide-resistant gene als was introduced into maize inbred line Qi319 through pollen-tube pathway. The inheritance of transgene was studied up to T3 generation. Transformation was performed by applying plasmid DNA carrying solution on the severed styles after self-pollination, or just prior to self-pollination. e confirmed positive in T0 generation, and sixteen of them were self-bred to construct family to T3 progeny. Through PCR analysis and herbicide screening in offsn other lines the numbers of positive plants were lower than expected. Therefore,it is necessary to select large population in progeny to produce stable inherited lines when transgene was delivered by pollen tube method.