| 自由和固定化猪胰脂肪酶在有机相中催化丁酸甲酯和四氢糠醇的酯交换反应 |
| |
| 引用本文： | 谢志东,暴奉维,何炳林,吕宪禹,云希勒,寇登民. 自由和固定化猪胰脂肪酶在有机相中催化丁酸甲酯和四氢糠醇的酯交换反应[J]. 南开大学学报(自然科学版), 1996, 0(4) |
| |
| 作者姓名： | 谢志东 暴奉维 何炳林 吕宪禹 云希勒 寇登民 |
| |
| 作者单位： | 南开大学高分子化学研究所，南开大学生命科学院 |
| |
| 摘 要： | 本文用自由和聚苯乙烯载体固定化猪胰脂肪酶在有机相中催化丁酸甲醋和四氢糠醇的酯交换反应．研究了不同有机溶剂、底物浓度、含水量、加酶量、温度及振荡速度等对反应的影响，对比了酶固定化前后的催化性能．结果表明，用疏水性交联聚苯乙烯载体固定化猪胰脂肪酸能减少扩散限制，固定化酶在有机相中催化活性比自由酶高约２５％．
|
| 关 键 词： | 猪胰脂肪酶；酯交换反应；固定化酶；有机相 |
TRANSESTERIFICATION OF METHYL BUTYRATE AND TETRAHYDROFURFURYL ALCOHOL CATALYZED BY FREE AND IMMOBILIZED LIPASE IN ORGANIC SYSTEMS |
| |
| Xie Zhidong, Bac Fengwei,He Binglin. TRANSESTERIFICATION OF METHYL BUTYRATE AND TETRAHYDROFURFURYL ALCOHOL CATALYZED BY FREE AND IMMOBILIZED LIPASE IN ORGANIC SYSTEMS[J]. Acta Scientiarum Naturalium University Nankaiensis, 1996, 0(4) |
| |
| Authors: | Xie Zhidong Bac Fengwei He Binglin |
| |
| Abstract: | Transesterification of methyl butyrate and tetrahydrofurfuryl alcohol in organic systems was catalyzed by free and immobilized lipase. Organic solvents,water content, content of lipase, substrate concetration and other parameters which affected the reaction were studied. It was found hydrophobic carrier which was used to immobilize lipase can reduce diffusion limitation,and the catalytic activity of immobilized lipase was 25% higher than that of free lipase. |
| |
| Keywords: | porcine pancreatic lipase transesterification immobilized enzyme organic systems |
| 本文献已被 CNKI 等数据库收录！ |
