大鼠大脑中动脉供血区梗死后小脑皮质GFAP mRNA动态表达

采用Longa大鼠大脑中动脉内栓线阻断法制备动物模型,用实时定量PCR法检测大鼠大脑中动脉闭塞后小脑皮质胶质纤维酸性蛋白mRNA的表达。结果显示,持续性大鼠大脑中动脉闭塞后,胶质纤维酸性蛋白mRNA在大鼠的小脑皮层有动态表达,第1天、第3天和第5天比对照组明显增高(P0.05),第7天、第10天和第14天比对照组明显下降(P0.05)。大鼠大脑中动脉闭塞后小脑皮质出现胶质纤维酸性蛋白动态表达,远离梗死灶的相关部位脑组织继发损害机制可能与神经抑制因子有关。

Dynamic Expression of GFAP mRNA of Cerebellar Cortex after the Infarction of Middle Cerebral Artery Supply Area in Rats

The changes of glial fibrillary acid protein（GFAP） mRNA in cerebellar cortex were observed after rat middle cerebral artery occlusion.The animal model was prepared with Longa＇s Bolt line blocking method（MCAO） in middle cerebral artery of rats,and the expression of GFAP mRNA was examined with real-time quantilative PCR method.The results show,GFAP mRNA has dynamic expression in the cerebellar cortex after persistent middle cerebral artery occlusion,The expression in the operated group significantly increases on the 1st,3nd and 5th day,but decreases on the 7th,10th and 14th day in compcerison with that of the sham-operated group（P0.05）.After rat middle cerebral artery occlusion,there are dynamic changes of GFAP in cerebellar cortex,which occurs far away from the site of infarction and secondary damage to brain tissue may be related to the neural inhibitory factor.