沼水蛙皮肤抗菌肽Temporin—HZ的结构和功能研究 |
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引用本文: | 黄颖桢,;周建武,;张洋,;饶平凡. 沼水蛙皮肤抗菌肽Temporin—HZ的结构和功能研究[J]. 莆田高等专科学校学报, 2008, 0(5): 30-34 |
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作者姓名: | 黄颖桢, 周建武, 张洋, 饶平凡 |
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作者单位: | [1]福州大学生物工程研究所,福建福州350002; [2]福建省农业科学院农业生物资源研究所药用植物研究中心,福建福州350003 |
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基金项目: | 国家自然科学基金资助项目(20475010) |
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摘 要: | 应用反相高效液相色谱和质谱联合使用手段,从沼水蛙皮肤分泌液中分离到一种新型的Temporin家族成员—Temporin-HZ,经过Edman降解氨基酸序列分析和串联质谱研究,Temporin-HZ的一级结构最终确定为Phe-Leu—Pro-Leu-Leu-Phe-Gla-Ala-Ile-Ser-His-Leu-Leu-NH2。抗菌活性分析表明,Temporin-HZ对大肠杆菌和白色念珠菌生长无明显抑制,但对金黄色葡萄球菌有抗菌作用。
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关 键 词: | 沼水蛙 抗菌肽 Temporin—HZ 测序 最小抑菌浓度 |
Structural Characterization and Structional Analysis of Temporin-HZ from Skin Secretion of Hylarana Guentheri |
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Affiliation: | HUANG Ying-zhen, ZHOU Jian-wu, ZHANG Yang, RAO Ping-fan ( 1. Institute ofBiotechnology, Fuzhou University, Fuzhou Fujian 350002, China; 2. Medicinal Plant Research Center, Institute of Agricultural Bioresources, Fujian Acedamy of Agricultural Science, Fuzhou Fujian 350003, China ) |
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Abstract: | A new temporin-HZ was isolated from the skin secretion of Hylarana guentheri by reverse phase HPLC and mass spectrometry analysis. The primary structure of temporin-HZ (Phe-Leu-Pro-Lcu-Lcu-Phe-Gla-Ala-Ile-Ser-His-Leu-Leu-NH2) was identified after Edman degradation amino acid sequencing and ms/ms Q-TOF analyzing. The tempofin-HZ had moderate activity against Staphylococcus aureus and no activity against Escherichia coli and Candida albicans. |
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Keywords: | Hylarana guentheri antimicrobial peptide temporin-HZ sequence minimal inhibitory concentration |
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