High resolution imaging using scanning ion conductance microscopy with improved distance feedback control |
| |
Authors: | Chao Li Nicholas Johnson Victor Ostanin Andrew Shevchuk Liming Ying Yuri Korchev David Klenerman |
| |
Institution: | 1. Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, United Kingdom 2. Division of Medicine, Imperial College London, MRC Clinical Sciences Centre, Hammersmith Campus, London W120NN, United Kingdom |
| |
Abstract: | Microscopy is an essential technique for observation on living cells. There is currently great interest in applying scanning probe microscopy to image-living biological cells in their natural environment at the nanometer scale. Scanning ion conductance microscopy is a new form of scanning probe microscopy, which enables non-contact high-resolution imaging of living biological cells. Based on a scanned nanopipette in physiological buffer, the distance feedback control uses the ion current to control the distance between the pipette tip and the sample surface. However, this feedback control has difficulties over slopes on convoluted cell surfaces, which limits its resolution. In this study, we present an improved form of feedback control that removes the contribution of up to the third-order slope from the ion current signal, hence providing a more accurate signal for controlling the distance. We show that this allows faster and lower noise topographic high-resolution imaging. |
| |
Keywords: | Living biological cells Scanning ion conductance microscopy Distance feedback control |
本文献已被 维普 万方数据 ScienceDirect 等数据库收录! |
| 点击此处可从《自然科学进展(英文版)》浏览原始摘要信息 |
| 点击此处可从《自然科学进展(英文版)》下载免费的PDF全文 |