The expression of vasa gene during zebrafish ( Danio rerio ) oogenesis |
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Authors: | Xiang Fang Zhen Yan Zheng Wen-xuan Deng Feng-jiao Wang Xiao-kai and Zhang Xi-yuan |
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Institution: | (1) Key Laboratory of MOE for Developmental Biology, College of Life Sciences, Wuhan University, 430072 Wuhan, Hubei, China;(2) Wuhan Municipal Fourth Hospital, 430033 Wuhan, Hubei, China |
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Abstract: | vasa gene expression pattern during oogenesis of zebrafish was examined usingin situ hybridization and fluorescent quantitative RT-PCR. During zebrafish oogensis,vasa mRNA is expressed strongly and uniformly distributed in the cytoplasm in stage II oocytes, followed by a distribution among
vacuome in stage III. Later in stage IV and V,vasa mRNA is enriched at the cortex and finally localized at the cortex. The fluorescent quantitative RT-PCR shows that the quantity
ofvasa mRNA decreases from stage II to stage III, but remains relatively invariable from stage III to stage V. The observed differences
invasa mRNA expression in the different stages of zebrafish oogenesis suggest thatvasa gene plays an important role during oogenesis.
Foundation item: Supported by the National Natural Science Foundation of China (30370744, 30150005)
Biography: XIANG Fang (1979-), male, Master candidate, research direction: molecular development of animals. |
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Keywords: | vasa zebrafish fluorescent quantitative RT-PCR oogenesis |
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