两面针中氯化两面针碱对照品的制备

建立从两面针中制备氯化两面针碱对照品的方法,利用硅胶柱层析、重结晶和反相制备高效液相色谱对两面针的乙醇提取物进行分离纯化,以HPLC对氯化两面针碱进行纯度检查和含量测定,并通过UV、IR、MS、1 HNMR等对其进行结构确证。从两面针叶中分离、纯化出氯化两面针碱对照品,质量分数>99.0%。所建的制备方法简单,制备出的氯化两面针碱对照品符合中药化学对照品的相关要求,可作为两面针药材和含两面针成药质量控制,以及中药药效物质基础用的化学对照品。

Preperation of Reference Substance of Nitidine Chloride from Zanthoxylum nitidum (Roxb.)DC.

To establish a seperation method for reference substance of nitidine chloride from leave of Zanthoxylum nitidum (Roxb.)DC., ethanol-extract of the leave of Zanthoxylum nitidum (Roxb.) DC.was isolated and purified by RP-HPLC combining with column chromatography and recrystallization.Its structure was identified by means of UV, IR, MS and ¹HNMR.The nitidine chloride was completely seperated from the leave of Zanthoxylum nitidum (Roxb.)DC., and the purity of the reference substance was no less than 99.0%.The method is simple and convenient, and the compound prepared by the above-mentioned method is accorded with the relative demands of chemical reference substance in Chinese materia medica.It can be used as a reference subsatance for the quality control and the research of herbal medicine.