Hormonal facilitation in the release of sperm from the spermatheca of the red-spotted newt |
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Authors: | M P Hardy J N Dent |
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Institution: | (1) Department of Biology, Gilmer Hall, University of Virginia, 22901 Charlottesville, Virginia, USA;(2) Present address: Department of Population Dynamics, Johns Hopkins School of Hygiene and Public Health, 21205 Baltimore, Maryland, USA |
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Abstract: | Summary Several neurotransmitters and hormones with potential to trigger a simultaneous contraction of the oviducts and the spermathecal myoepithelium were examined. Saline (0.05 ml), or 0.05 ml saline plus acetylcholine (9 mg), norepinephrine (50 g), arginine-vasotocin (25 units), prostaglandin F2(3g) were injected into the spermathecal region of female newts (n=24 per group). The numbers of sperm present in the cloacae of prostaglandin-injected animals (107±30 SEM) were significantly greater than the numbers detected in saline (27±5 SEM) and in uninjected (14±3 SEM) controls. Smaller and less consistent increases in the numbers of sperm were detected in the vasotocin- and norepinephrine-injected groups. Study of sections from ovulating female newts failed to produce evidence that pressure from the passage of ova through the posterior portion of the oviduct forced sperm from the spermatheca. Observations indicate an active role for the spermathecal myoepithelium in the discharge of stored sperm and of a role for prostaglandin F2 in triggering that discharge. |
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Keywords: | Spermatheca newt spermatheca sperm storage sperm discharge from storage hormonal action in sperm discharge prostaglandin and sperm discharge |
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