Calcium release from frog sarcoplasmic reticulum by an imidazolyl reagent |
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| Authors: | T Aoki T Oba |
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| Institution: | Department of Physiology, Nagoya City University Medical School, Japan. |
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| Abstract: | Calcium is released from the isolated heavy sarcoplasmic reticulum (SR) of frog skeletal muscle upon application of 0.1-1 mM diethylpyrocarbonate (DEP, an imidazolyl reagent). The Ca-ATPase activity of SR was suppressed by 20% in the presence of 1 mM DEP. More than 1 mM of free magnesium ion or 5 microM ruthenium red eliminated the effect of DEP on calcium release but not on Ca-ATPase activity. A plausible site of DEP action is on the calcium channel. |
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