| Transgene directionally integrated into C-genome of Brassica napus |
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| 引用本文： | LI Jun FANG Xiaoping WANG Zhuan LUO Lixia HU Qiong. Transgene directionally integrated into C-genome of Brassica napus[J]. 科学通报(英文版), 2006, 51(13): 1578-1585. DOI: 10.1007/s11434-006-2021-4 |
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| 作者姓名： | LI Jun FANG Xiaoping WANG Zhuan LUO Lixia HU Qiong |
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| 作者单位： | Key Laboratory of Oil Crops Genetic Improvement of the Ministry of Agriculture, Institute of Oil Crops Research, Chinese Academy of Agricultural Sciences, Wuhan 430062, China |
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| 基金项目： | Acknowledgements We thank Dr. Lu Changming and Dr. Lu Guangyuan for modification opinions of the paper. This work was supported by National 863 Project （Grant Nos. 2002AA212011, 2003AA222101 ＆ 2005AA241030） and the National Natural Science Foundation of China （Grant No. 30270791 ）. |
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| 摘 要： | Transgenic Brassica napus has been widely planted in Canada, the United States, and some other countries. In China, although the policy for genetically modified foods has not yet opened, genetically modified rape- seed oil as raw material for biodiesel of…
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| 关 键 词： | C-染色体组 Bar基因 定向转基因 芸苔 再合成 |
| 收稿时间： | 2006-03-14 |
| 修稿时间： | 2006-03-142006-04-17 |
Transgene directionally integrated into C-genome of Brassica napus |
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| Jun Li,Xiaoping Fang,Zhuan Wang,Jun Li,Lixia Luo,Qiong Hu. Transgene directionally integrated into C-genome of Brassica napus[J]. Chinese science bulletin, 2006, 51(13): 1578-1585. DOI: 10.1007/s11434-006-2021-4 |
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| Authors: | Jun Li Xiaoping Fang Zhuan Wang Jun Li Lixia Luo Qiong Hu |
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| Affiliation: | (1) Key Laboratory of Oil Crops Genetic Improvement of the Ministry of Agriculture, Institute of Oil Crops Research, Chinese Academy of Agricultural Sciences, Wuhan, 430062, China |
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| Abstract: | Integration of a transgene into a C-genome chromosome plays an important role in reducing ecological risk of transgenic Brassica napus To obtain C-genome transgenic B. napus, herbi- cide-resistant bar gene was firstly transferred into B. oleracea var. alboglabra mediated by Agrobacterium tumefaciens strain LBA4404. Then using the trans- genic B. oleracea as paternal plants and 8 non- transgenic varieties of B. rapa as maternal plants, C- genome transgenic B. napus with bar gene was arti- ficially resynthesized by means of ovary culture and chromosome doubling. Among 67 lines of the resyn- thesized B. napus, 31 were positive, and 36 were negative according to PCR test for bar gene. At least 2 plants from each line were kept for PPT spray con- firmation. The result was in consistence with the PCR test. Genomic Southern blotting of three randomly chosen lines also showed that bar gene had been integrated into the genome of resynthesized B. napus lines. |
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| Keywords: | Bar gene C-genome directional transgene resynthesis of Brassica napus. |
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