基于NMR的维吾尔医异常黑胆质型糖尿病代谢组学研究

 研究异常黑胆质型糖尿病患者体内物质代谢变化的规律。采用基于核磁共振(NMR)测定技术对异常黑胆质型糖尿病患者和黑胆质血糖正常者尿液进行检测,使用正交偏最小二乘法判别(Orthogonal Partial Least-Squares Discriminant Analysis, OPLS-DA)分析研究尿液内源性化合物在两组间的差异。结果显示,异常黑胆质型糖尿病患者组尿液中的代谢物发生了明显的变化。其中葡萄糖、牛磺酸和甜菜碱的含量增加,与黑胆质血糖正常组比较差异有统计学意义(P<0.05);同时多种氨基酸、肌酸酐、马尿酸盐、柠檬酸和二甲胺等含量减少,与黑胆质血糖正常组比较差异有统计学意义(P<0.05)。总之,基于NMR的代谢组学技术和标志物信息均显示异常黑胆质型糖尿病患者和黑胆质血糖正常者存在代谢差异,提示体内某些小分子化合物可能正是维吾尔医异常黑胆质型糖尿病的物质基础。

Metabonomic Investigation on Abnormal Savda Patients with Type Two Diabetes Mellitus Using NMR Spectroscopy

Based on the theory of Traditional Uighur Medicine, the metabolites changes in the urine of patients of type two diabetes mellitus with abnormal savda are investigated. 1H Nuclear Magnetic Resonance (NMR) based metabonomic analysis is performed on the urine samples obtained from both abnormal savda patients of type two diabetes mellitus and savda volunteers with normal plasma glucose. The Orthogonal Partial Least-Squares Discriminant Analysis (OPLS-DA) is conducted to distinguish the metabolic phenotypes. The results show that the metabolites in the urine of the patients of type two diabetes mellitus with abnormal savda are significantly changed. The concentrations of glucose, taurine, and betaine are increased (P<0.05); Meanwhile, the concentrations of some amino acids, creatinine, hippurate, citric acid, and dimethylamine are decreased (P<0.05) comparing with savda volunteers with normal plasma glucose. The fact indicates that there are great differences in the urine metabolites between the patients of type two diabetes mellitus with abnormal savda and savda volunteers with normal plasma glucose by using NMR. And it also reveals that endogenous compounds might be the ingredients for the patients of type two diabetes mellitus with abnormal savda.