EC109细胞株细胞周期DNA代谢和形态改变的研究

人食管癌细胞株 EC109以显微分光光度计测定癌细胞核 DNA 含量,以放射自显影测定细胞核摄取~3H-TdR 和扫描电镜检查细胞表面的超微结构.大多数 EC109瘤细胞间期细胞核的 DNA 含量增加,主要干系细胞 DNA 含量为2 C,属 G_1期,也可见低于四倍体(3 C),高于四倍体(>4 C).甚至多倍体(>8 C)的瘤细胞.~3H-TdR 加入培养基10分钟后,许多瘤细胞核有银粒出现(S 期细胞).大量细胞进入细胞周期反映其恶性程度高.扫描电镜可见 EC109在细胞周期不同阶段其细胞大小形状和微绒毛也不同.本文结果说明用细胞核 DAN 含量测定,~3H-TdR 掺入和细胞表面超微结构可测定肿瘤周期.也可用这些方法判定瘤细胞的恶性程度.

Study on the DNA Metabolic and Morphologic Changes in Cell Cycle of EC-109 Cell Line

Human esophageal carcinoma cell line,EC109,was examined for the nuclear DNA content by microspectrophotometry,for its ~3H-TdR uptake by radioautography,and for its superficial ultrastructure by scanning electronic microscopy.The DNA content in the interphase nuclei of most EC109 tumor cells were increased,and although the stem line correlated well with the relative DNA content (2C) of G_1 population,the hypotetraploid (3C),hypertetraploid (>4C) and polyploid (>8C)population of tumor cells appeared also.The silver grains appeared in many nuclei of tumor cells in S phase within 10 minutes after adding ~3H-TdR into culture medium.A large amount of tumor cells entering cell cycle reflected a hypermalignan- cy.Under the scanning electronic microscope,the superficial ultrastruc- tufts of the EC109 were different in their size,shape and microvilli at different phases of the cell cycle. The investigation showed that the distribution of tumor cells in the various phases of cell cycle can be determined by measuring DNA con- tent,~3H-TdR uptake and superficial ultrastructure,and that the malignan- cy of the tumor could also be determined by these methods.