| IGF-Ⅱ and IGFBP-1 reversely regulate blastocyst implantation in mouse |
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| 作者单位： | TIAN Yongqiang(State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China;College of Vet Medicine, Gansu Agricultural University, Lanzhou 730070, China);TIE Guodong,CAO Yujing,DUAN Enkui(State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China);ZHAO Xingxu(College of Vet Medicine, Gansu Agricultural University, Lanzhou 730070, China) |
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| 基金项目： | 国家重点基础研究发展计划(973计划)，中国科学院"百人计划" |
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| 摘 要： | Insulin-like growth factor (IGF)-Ⅱ and IGF binding protein (IGFBP)-1, members of IGF family, are important in the cyclic development of endometrium and the blastocyst implantation. In the present study, the indirect immunofluorescence showed that IGF-Ⅱ and IGFBP-1 were specifically expressed at the maternal-fetal interface. In a co-culture system, IGF-Ⅱ significantly enhanced the attachment and outgrowth of the blastocyst on monolayer of uterine epithelial cells, while IGFBP-1 did not affect the blastocyst attachment, but markedly inhibited the blastocyst outgrowth. The results of zymography showed that IGF-Ⅱ enhanced the activities of MMP-2 and MMP-9, while IGFBP-1 did not affect the activities of MMP-2 and MMP-9. In conclusion, the equilibrium between the invasion of trophoblast and the inhibition of deciduas may be regulated by the interaction between the IGF-Ⅱ-expressing invading cytotrophoblast and maternal deciduas-derived IGFBP-1.
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| 关 键 词： | IGF-Ⅱ |
| 收稿时间： | 2002-07-24 |
IGF-II and IGFBP-1 reversely regulate blastocyst implantation in mouse |
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| Yongqiang Tian,Guodong Tie,Yujing Cao,Xingxu Zhao,Enkui Duan. IGF-II and IGFBP-1 reversely regulate blastocyst implantation in mouse[J]. Chinese science bulletin, 2002, 47(21): 1816-1820. DOI: 10.1007/BF03183849 |
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| Authors: | Yongqiang Tian Guodong Tie Yujing Cao Xingxu Zhao Enkui Duan |
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| Affiliation: | (1)State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080 ,China;
(2)College of Vet Medicine, Gansu Agricultural University, Lanzhou 730070 ,China |
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| Abstract: | Insulin-like growth factor (IGF)-Ⅱ and IGF binding protein (IGFBP)-1, members of IGF family, are important in the cyclic development of endometrium and the blastocyst implantation. In the present study, the indirect immunofluorescence showed that IGF-Ⅱ and IGFBP-1 were specifically expressed at the maternal-fetal interface. In a co-culture system, IGF-Ⅱ significantly enhanced the attachment and outgrowth of the blastocyst on monolayer of uterine epithelial cells, while IGFBP-1 did not affect the blastocyst attachment, but markedly inhibited the blastocyst outgrowth. The results of zymography showed that IGF-Ⅱ enhanced the activities of MMP-2 and MMP-9, while IGFBP-1 did not affect the activities of MMP-2 and MMP-9. In conclusion, the equilibrium between the invasion of trophoblast and the inhibition of deciduas may be regulated by the interaction between the IGF-Ⅱ-expressing invading cytotrophoblast and maternal deciduas-derived IGFBP-1. |
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| Keywords: | IGFBP-1 implantation MMP-2 MMP-9 |
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