| 反相高效液相色谱法分离三联吡啶衍生物 |
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| 引用本文： | 张晓燕,刘艳丽,李文霞. 反相高效液相色谱法分离三联吡啶衍生物[J]. 中州大学学报, 2012, 29(4): 106-108 |
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| 作者姓名： | 张晓燕 刘艳丽 李文霞 |
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| 作者单位： | 1. 洛阳市环境监测站,洛阳,471009
2. 中州大学实验管理中心,郑州,450044 |
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| 摘 要： | 采用反相高效液相色谱法对五种三联吡啶衍生物的混合物进行了分离。采用Symmetry C18分析柱(3.9mm i.d.×150mm,5μm),甲醇-水作为流动相,利用紫外检测器在280 nm处对各物质进行分离检测。当流动相组成为甲醇-水(8∶2),流速为0.8 mL/min时,各物质在15 min内均可以得到基线分离。五种三联吡啶衍生物在浓度为0.1～400μg/mL时线性关系良好,回归系数均大于0.9993,各组分相对标准偏差小于2.2%。
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| 关 键 词： | 反相高效液相色谱法 三联吡啶衍生物 分离 |
Separation of Terpyridine Derivatives by High-performance Liquid Chromatography |
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| ZHANG Xiao-yang,LIU Yan-li,LI Wen-xia. Separation of Terpyridine Derivatives by High-performance Liquid Chromatography[J]. Journal of Zhongzhou University, 2012, 29(4): 106-108 |
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| Authors: | ZHANG Xiao-yang LIU Yan-li LI Wen-xia |
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| Affiliation: | 1(1.The Environmental Monitoring Station of Luoyang,Henan Luoyang 471009,China; 2.Experiment Administration Center,Zhongzhou University,Zhengzhou 450044,China) |
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| Abstract: | A method was proposed for the separation and determination of five tepyridine derivatives by high performance liquid chromatography.The separation was carried out on a Symmetry C18(3.9mm i.d.×150mm,5μm) column with methanol-water(8:2) as mobile phase and detection wavelength(280nm) in room temperature,isotropic elution.Measurement could be finished in 15min with a good linearity for samples concentration range from 0.1μg/mL to 400μg/mL and the relative standard deviation below 2.2%.This method is simple,efficient,and accurate. |
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| Keywords: | reversed-phase high-performance liquid chromatography terpyridine derivatives separation |
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