上海市11种常规粳稻抗稻瘟病基因分子标记检测

利用分子标记检测技术,对9种参加2020年上海市水稻区域试验的品种和2种本课题组新培育的新品系的共10个抗稻瘟病基因位点进行了检测.结果显示,Pi37,Pi41,Pi-d23个基因在11种水稻中出现的频率达100％,Pi2,Pi5,Pi9,Pi36,Pikm和Pib抗性基因在11种水稻中出现的频率分别为18.18％,9...

Molecular marker detection of rice blast resistance genes in 11 Shanghai conventional japonica varieties

In this study, by using these known molecular markers together with PCR technology, 10 rice blast resistance gene loci were amplified in 9 new rice varieties that were involved in 2020 Shanghai Regional Trial and 2 rice lines that were developed in our laboratory. Our results indicated that the occurrence frequency of Pi37, Pi41, and Pi-d2 genes was 100%, while the distribution frequency of Pi2, Pi5, Pi9, Pi36, Pikm, Pib and Pigm genes in 11 tested rice varieties/lines was 18.18%, 9.09%, 36.36%, 90.9%, 9.09%, 81.81%, and 0, respectively. Collectively, the results of this study provided scientific evidence for pyramiding more blast resistant genes and breeding new rice varieties with broad-spectrum rice blast resistance.