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上海市11种常规粳稻抗稻瘟病基因分子标记检测
引用本文:谢水锋,宗士鹏,余飞宇,赵国超,李建粤. 上海市11种常规粳稻抗稻瘟病基因分子标记检测[J]. 上海师范大学学报(自然科学版), 2022, 51(1): 56-63
作者姓名:谢水锋  宗士鹏  余飞宇  赵国超  李建粤
作者单位:上海师范大学生命科学学院,上海200234;光明米业(集团)有限公司农业技术中心,上海202171
基金项目:上海市科委现代农业科技创新项目(19391900500)
摘    要:利用分子标记检测技术,对9种参加2020年上海市水稻区域试验的品种和2种本课题组新培育的新品系的共10个抗稻瘟病基因位点进行了检测.结果显示,Pi37,Pi41,Pi-d23个基因在11种水稻中出现的频率达100%,Pi2,Pi5,Pi9,Pi36,Pikm和Pib抗性基因在11种水稻中出现的频率分别为18.18%,9...

关 键 词:水稻(Oryza sativa)  抗稻瘟病基因  分子标记
收稿时间:2021-12-02

Molecular marker detection of rice blast resistance genes in 11 Shanghai conventional japonica varieties
XIE Shuifeng,ZONG Shipeng,YU Feiyu,ZHAO Guochao,LI Jianyue. Molecular marker detection of rice blast resistance genes in 11 Shanghai conventional japonica varieties[J]. Journal of Shanghai Normal University(Natural Sciences), 2022, 51(1): 56-63
Authors:XIE Shuifeng  ZONG Shipeng  YU Feiyu  ZHAO Guochao  LI Jianyue
Affiliation:College of Life Sciences, Shanghai Normal University, Shanghai 200234, China;Agricultural Technology Center of Guangming Rice(Group) Co., Ltd., Shanghai 202171, China
Abstract:In this study, by using these known molecular markers together with PCR technology, 10 rice blast resistance gene loci were amplified in 9 new rice varieties that were involved in 2020 Shanghai Regional Trial and 2 rice lines that were developed in our laboratory. Our results indicated that the occurrence frequency of Pi37, Pi41, and Pi-d2 genes was 100%, while the distribution frequency of Pi2, Pi5, Pi9, Pi36, Pikm, Pib and Pigm genes in 11 tested rice varieties/lines was 18.18%, 9.09%, 36.36%, 90.9%, 9.09%, 81.81%, and 0, respectively. Collectively, the results of this study provided scientific evidence for pyramiding more blast resistant genes and breeding new rice varieties with broad-spectrum rice blast resistance.
Keywords:rice (Oryza sativa)  blast resistance genes  molecular marker
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