| 上海市11种常规粳稻抗稻瘟病基因分子标记检测 |
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| 引用本文： | 谢水锋,宗士鹏,余飞宇,赵国超,李建粤.上海市11种常规粳稻抗稻瘟病基因分子标记检测[J].上海师范大学学报(自然科学版),2022,51(1):56-63. |
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| 作者姓名： | 谢水锋 宗士鹏 余飞宇 赵国超 李建粤 |
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| 作者单位： | 1. 上海师范大学生命科学学院;2. 光明米业(集团)有限公司农业技术中心 |
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| 基金项目： | 上海市科委现代农业科技创新项目（19391900500） |
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| 摘 要： | 利用分子标记检测技术,对9种参加2020年上海市水稻区域试验的品种和2种本课题组新培育的新品系的共10个抗稻瘟病基因位点进行了检测.结果显示,Pi37,Pi41,Pi-d23个基因在11种水稻中出现的频率达100％,Pi2,Pi5,Pi9,Pi36,Pikm和Pib抗性基因在11种水稻中出现的频率分别为18.18％,9...
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| 关 键 词： | 水稻(Oryza sativa) 抗稻瘟病基因 分子标记 |
| 收稿时间： | 2021/12/2 0:00:00 |
Molecular marker detection of rice blast resistance genes in 11 Shanghai conventional japonica varieties |
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| XIE Shuifeng,ZONG Shipeng,YU Feiyu,ZHAO Guochao,LI Jianyue.Molecular marker detection of rice blast resistance genes in 11 Shanghai conventional japonica varieties[J].Journal of Shanghai Normal University(Natural Sciences),2022,51(1):56-63. |
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| Authors: | XIE Shuifeng ZONG Shipeng YU Feiyu ZHAO Guochao LI Jianyue |
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| Institution: | College of Life Sciences, Shanghai Normal University, Shanghai 200234, China;Agricultural Technology Center of Guangming Rice(Group) Co., Ltd., Shanghai 202171, China |
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| Abstract: | In this study, by using these known molecular markers together with PCR technology, 10 rice blast resistance gene loci were amplified in 9 new rice varieties that were involved in 2020 Shanghai Regional Trial and 2 rice lines that were developed in our laboratory. Our results indicated that the occurrence frequency of Pi37, Pi41, and Pi-d2 genes was 100%, while the distribution frequency of Pi2, Pi5, Pi9, Pi36, Pikm, Pib and Pigm genes in 11 tested rice varieties/lines was 18.18%, 9.09%, 36.36%, 90.9%, 9.09%, 81.81%, and 0, respectively. Collectively, the results of this study provided scientific evidence for pyramiding more blast resistant genes and breeding new rice varieties with broad-spectrum rice blast resistance. |
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| Keywords: | rice (Oryza sativa) blast resistance genes molecular marker |
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