基于表型性状的菠菜核心种质构建

以483份菠菜种质为材料,基于20个表型性状数据,根据2种遗传距离、3种取样方法、8种系统聚类方法、3种取样比例构建了144份菠菜核心种质.应用均值差异百分率、方差差异百分率、极差符合率和变异系数变化率4个参数来检验各取样策略的优劣.通过比较核心种质和原始种质的变异系数、方差、多样性指数、符合率和主成分,对构建的核心种...

Construction of spinach's core germplasms based on its phenotypic traits

This study used 483 spinach germplasm as materials, based on 20 phenotypic traits, and based on 2 genetic distances, 3 sampling methods, and 8 systematic clustering method and 3 sampling ratios constructed 144 core collections of spinach. Four parameters, the percentage of mean difference, the percentage of variance difference, the coincidence rate of range, and the rate of variation of the coefficient of variation, were used to test the pros and cons of each sampling strategy. By comparing the coefficient of variation, variance, diversity index, coincidence rate and principal components of the core collection and the original collection, the representative test of the constructed core collection was carried out. The results show that:"Euclidean distance+priority sampling method+deviation square sum method+15% sampling ratio" sampling strategy is the best, and its mean difference percentage, variance difference percentage, range coincidence rate, and variation coefficient change rate are 0, 65%, 100%, and 124.32% respectively. The average coincidence rate of the core collection and the original collection on the 20 indicators is between 95.08% and 99.83%, the maximum and minimum coincidence rates are both 100%, and the diversity index coincidence rate is between 83.94% and 99.11%. The first six principal components of the original germplasm and the core germplasm are same, and the cumulative contribution rates are 69.563% and 70.242%, respectively. It shows that the 72 spinach core collections constructed can represent the phenotypic variation characteristics of 483 original collections to be utilized and preserved.