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Investigation of the effects of copper ions on protein aggregation using a model system
Authors:C Capanni  N Taddei  S Gabrielli  L Messori  P Orioli  F Chiti  M Stefani  G Ramponi
Institution:(1) Dipartimento di Scienze Biochimiche, Università di Firenze, Viale Morgagni 50, 50134 Firenze, Italy;(2) Dipartimento di Chimica, Polo Scientifico, Università di Firenze, Sesto F.no, Firenze, Italy
Abstract:Protein aggregation is a notable feature of various human disorders, including Parkinsonrsquos disease, Alzheimerrsquos disease and many others systemic amyloidoses. An increasing number of observations in vitro suggest that transition metals are able to accelerate the aggregation process of several proteins found in pathological deposits, e.g. agr-synuclein, amyloid beta (Abeta) peptide, beta2-microglobulin and fragments of the prion protein. Here we report the effects of metal ions on the aggregation rate of human muscle acylphosphatase, a suitable model system for aggregation studies in vitro. Among the different species tested, Cu2+ produced the most remarkable acceleration of aggregation, the rate of the process being 2.5-fold higher in the presence of 0.1 mM metal concentration. Data reported in the literature suggest the possible role played by histidine residues or negatively charged clusters present in the amino acid sequence in Cu2+-mediated aggregation of pathological proteins. Acylphosphatase does not contain histidine residues and is a basic protein. A number of histidine-containing mutational variants of acylphosphatase were produced to evaluate the importance of histidine in the aggregation process. The Cu2+-induced acceleration of aggregation was not significantly altered in the protein variants. The different aggregation rates shown by each variant were entirely explained by the changes of hydrophobicity or propensity to form a beta structure introduced by the point mutation. The effect of Cu2+ on acylphosphatase aggregation cannot therefore be attributed to the specific factors usually invoked in the aggregation of pathological proteins. The effect, rather, seems to be a general related to the chemistry of the polypeptide backbone and could represent an additional deleterious factor resulting from the alteration of the homeostasis of metal ions in cells.Received 4 December 2003; received after revision 7 January 2004; accepted 3 February 2004
Keywords:Protein aggregation  protein misfolding  amyloid fibrils  Cu2+  metal ions
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