| 鸡传染性法式囊病毒VP2原核表达蛋白的纯化和复性 |
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| 引用本文： | 王慧杰,杨向科.鸡传染性法式囊病毒VP2原核表达蛋白的纯化和复性[J].河南科学,2006,24(5):687-690. |
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| 作者姓名： | 王慧杰 杨向科 |
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| 作者单位： | 郑州牧业工程高等专科学校,郑州,450011 |
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| 摘 要： | 对原核表达的鸡传染性法式囊病毒(IBDV)VP2蛋白进行可溶性分析与不可溶性分析,结果表明蛋白主要以包涵体形式存在,利用溶菌酶、Triton-100、尿素等试剂进行纯化和复性后,对复性前后的蛋白分别与特异性多抗进行Dot-ELISA,复性后蛋白的反应活性增强了10倍,对复性后的蛋白与IBDV16株单抗进行Dot-ELISA,其中有11株与其发生特异性反应.
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| 关 键 词： | 鸡传染性法式囊病毒 VP2蛋白 包涵体 纯化 复性 |
| 文章编号： | 1004-3918(2006)05-0687-04 |
| 收稿时间： | 2006-04-01 |
| 修稿时间： | 2006年4月1日 |
Purification and Refold of IBDV Structural Protein VP2 Expressed in Escherichia Coli |
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| WANG Hui-jie,YANG Xiang-ke.Purification and Refold of IBDV Structural Protein VP2 Expressed in Escherichia Coli[J].Henan Science,2006,24(5):687-690. |
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| Authors: | WANG Hui-jie YANG Xiang-ke |
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| Abstract: | IBDV structural protein VP2 expressed in Escherichia coli was analysed by soluble and insoluble, the result showed that protein was produced in the form of insoluble bodies. Then the protein were sublimated and refolded by lysozyme, Triton-100, carbamide, which the activity of the protein was increased 10 time by Dot-ELISA with polyclonal antibodies compared with protein before purification. Refolded protein were assayed with 16 monoclonal antibodies by Dot-ELISA, which 11 monoclonal antibodies were reactivity. |
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| Keywords: | infectious bursal disease virus VP2 structural protein inclusion body purification refold |
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