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Expression of functional acetylcholine receptor from cloned cDNAs |
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| Authors: | M Mishina T Kurosaki T Tobimatsu Y Morimoto M Noda T Yamamoto M Terao J Lindstrom T Takahashi M Kuno |
| Abstract: | The cloned cDNAs encoding the four subunits of the Torpedo californica acetylcholine receptor, each carried by a simian virus 40 vector, direct the synthesis of the functional receptor in a combined expression system consisting of COS monkey cells and Xenopus oocytes. Our results suggest that all four subunits are required to elicit a normal nicotinic response to acetylcholine, whereas only the alpha-subunit is indispensable for alpha-bungarotoxin binding activity. |
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