| Direct observation of the clockwise light-driven rotation of F_0F_(1-)ATP synthase complex |
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| 作者姓名： | CUI Yuanbo ZHANG Yinghao YUE Jiachang & JIANG Peidong
The National Laboratory of Biomacromolecules Institute of Biophysics Chinese Academy of Sciences Beijing China
The Graduate School of Chinese Academy of Sciences Beijing China |
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| 作者单位： | CUI Yuanbo，ZHANG Yinghao，YUE Jiachang & JIANG Peidong
The National Laboratory of Biomacromolecules，Institute of Biophysics，Chinese Academy of Sciences，Beijing 100101，China
The Graduate School of Chinese Academy of Sciences，Beijing
100039，China |
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| 摘 要： | The purified thermophilic bacterium PS3 F1βP10×His-tag is inserted into the F0F1-ATP synthases of chro matophores isolated from photosynthetic bacteria Rhodo spirillum rubrum. The studies of biochemical properties of the hybrid chromatophores show that they have both protons-driving capability and photophosphorylation. The fluorescent actin filaments, as a marker of its orientation by video-microscopic experiment, are connected via Maleimido C3-NTA to the reconstituted β10×His-tag of F0F1-ATP synthases. The clockwise rotation of F0F1-ATP synthases driven by light is observed directly when viewed from the F0 side to F1- This system should be valuable for further studying the coupling property of F0F1-ATP synthase.
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Direct observation of the clockwise light-driven rotation of F_0F_(1-)ATP synthase complex |
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| CUI Yuanbo,ZHANG Yinghao,YUE Jiachang & JIANG Peidong
The National Laboratory of Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,Beijing ,China
The Graduate School of Chinese Academy of Sciences,Beijing
,China.Direct observation of the clockwise light-driven rotation of F_0F_(1-)ATP synthase complex[J].Chinese Science Bulletin,2004(13). |
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| Authors: | CUI Yuanbo ZHANG Yinghao YUE Jiachang & JIANG Peidong
The National Laboratory of Biomacromolecules |
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| Institution: | CUI Yuanbo,ZHANG Yinghao,YUE Jiachang & JIANG Peidong
The National Laboratory of Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China
The Graduate School of Chinese Academy of Sciences,Beijing
100039,China |
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| Abstract: | |
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| Keywords: | photosynthetic bacteria chromatophores F0F1-ATP syn thase molecular motor light-driven clockwise rotation |
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