Chemical studies on the silver staining of nucleoli |
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Authors: | H Kling L Lepore W Krone J Olert G Sawatzki |
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Institution: | (1) Abt. Humangenetik and Abt. für Transfusionsmedizin, Universität Ulm, P.O. Box 4066, D-7900 Ulm, (Federal Republic of Germany) |
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Abstract: | Summary The uptake of Ag-ions by isolated nucleoli of rat liver cells was studied. Nucleolar proteins separated by electrophoresis were examined for selective silver-staining. A mechanism for a preferential Ag-staining of the nucleoli is discussed.We thank Mrs L. Schwinn for her valuable technical assistance, and Mrs K. Jain for presenting us DNA. We would like to thank the Department of Biology V for making possible the measurements with the atomic absorption spectrophotometer. This work was supported by the Deutsche Forschungsgemeinschaft. |
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