Ca2+-dependence and metabolic status of an obligate thermophile,Thermoactinomyces vulgaris,under shake culture conditions

Summary Ca²⁺ stimulates germination ofT. vulgaris spores. There is a higher mycelial yield as well as higher protein, DNA, RNA and free Pi content in cultures grown in the presence of Ca²⁺ as compared to those grown in the absence of this divalent cation.Grateful thanks are due to Professor R. P. Roy for providing the necessary facilities. V. P. S. acknowledges with thanks the receipt of a Post Doctoral Research Fellowship of the Council of Scientific and Industrial Research, New Delhi.