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| 固定化微环境对南方红豆杉细胞生长 | |
| 引用本文： | 尹德明,元英进,娄红祥.固定化微环境对南方红豆杉细胞生长[J].山东大学学报(理学版),2007,42(1):64-68. |
| 作者姓名： | 尹德明 元英进 娄红祥 |
| 作者单位： | 1. 山东大学,药学院天然药物化学教研室,山东,济南,250012;天津大学,化工学院制药工程系,天津,300072 2. 天津大学,化工学院制药工程系,天津,300072 3. 山东大学,药学院天然药物化学教研室,山东,济南,250012 |
| 摘 要： | 研究了固定化微环境对南方红豆杉细胞生长和紫杉醇合成的影响，结果表明：固定化细胞停滞期缩短，细胞膜脂氧合酶活性和脂过氧化产物含量远远高于悬浮细胞，同时苯丙氨酸解氨酶活性显著增加，微环境溶液内紫杉醇含量与悬浮细胞有显著性差异. 研究表明固定化微环境能促进红豆杉细胞紫杉醇含量增加但对细胞膜脂产生了过氧化. |
| 关 键 词： | 固定化 微环境 南方红豆杉细胞 紫杉醇 |
| 文章编号： | 1671-9352（2007）01-0064-05 |
| 收稿时间： | 2006-07-10 |
| 修稿时间： | 2006-07-10 |
Influences of immobilization microenvironment on Taxus chinensis var.mairei cell growth and taxol synthesis |
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| YIN De-ming,YUAN Ying-jin,LOU Hong-xiang.Influences of immobilization microenvironment on Taxus chinensis var.mairei cell growth and taxol synthesis[J].Journal of Shandong University,2007,42(1):64-68. | |
| Authors: | YIN De-ming YUAN Ying-jin LOU Hong-xiang |
| Institution: | 1, School of Pharmacetical Sciences, Shandong Univ., Jinan 250012, Shandong, China; 2. School of Chemical Engineering and Technology, Tianjin Univ,, Tianjin 300072, China |
| Abstract: | Cell growth and taxol synthesis of Taxus chinensis var.mairei were investigated under the immobilization microenvironment.The results show that the stagnation period of immobilized-cell is shortened.Lipoxygenase activity and malondialdehyde content of immobilized-cell are significantly higher than those of suspended-cell.Both phenylalanine ammonia-lyase activity of immobilized-cell and extracelluar taxol content in the microenvironment show a marked increase.An immobilization microenvironment can promote taxol content and peroxidize the cell membrane lipid. |
| Keywords: | immobilization microenvironment Taxus chinensis var mairei cells taxol |
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