|
|||||
|
|
| 蒜头果中一种新的植物蛋白(malanin)的分离纯化 | |
| 引用本文： | 袁燕,戴晓畅,段玲燕,陈雪,岑晓江.蒜头果中一种新的植物蛋白(malanin)的分离纯化[J].云南大学学报(自然科学版),2009,31(4):428-432. |
| 作者姓名： | 袁燕 戴晓畅 段玲燕 陈雪 岑晓江 |
| 作者单位： | 1. 云南大学教育部自然资源药物化学重点实验室, 云南昆明 650091; 2. 昆明学院生命科学与技术系, 云南昆明650031; 3. 昆明贝克诺顿制药股份有限公司云南昆明650100 |
| 摘 要： | 蒜头果(Malania oleifera Chunet S.Lee)属于铁青树科(Olacaceae)蒜头果属.蒜头果蛋白(malanin)是通过粉碎、抽提、硫酸铵沉淀、疏水色谱层析等分离手段,从蒜头果种仁中分离、纯化出的一种新的植物蛋白.Malanin是一种糖蛋白,分子质量为61875u,等电点为5.5,由A,B2条肽链通过二硫键连接而成.该蛋白的A链和B链N-端序列分别为DYPKLTFTTS和DETXTDEEFN(X一般情况下为C),用NCBI-BLASTP程序对nr数据库和其它蛋白质数据库进行检索,未发现有相同的序列存在.经基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)及分析各自的肽质量指纹图谱(PMF),结果没有匹配数据库中的任何记录,说明malanin为新蛋白质. |
| 关 键 词： | 蒜头果 蒜头果蛋白 分离纯化 |
| 收稿时间： | 2009-3-30 |
Isolation and purification of a novel plant protein from Malania oleifera |
|
| YUAN Yan,DAI Xiao-chang,DUAN Ling-yan,CHEN Xue,CEN Xiao-jiang.Isolation and purification of a novel plant protein from Malania oleifera[J].Journal of Yunnan University(Natural Sciences),2009,31(4):428-432. | |
| Authors: | YUAN Yan DAI Xiao-chang DUAN Ling-yan CHEN Xue CEN Xiao-jiang |
| Institution: | 1. Key Laboratory of Medical Chemistry for Natural Resource, Ministry of Education, School of Chemical Science and Technology, Yunnan University, Kunming 650091, China; 2. Department of Life Science and Technology, Kunming University, Kunming 650031, China; 3. Kunming Baker Norton Pharmaceutical Co.Ltd, Kunming 650100, China |
| Abstract: | Malania oleifera Chun et S.Lee belongs to Olacaceae.Anovel protein,designated as malanin,was isolated and purified from the seeds of Malania oleifera by homogenization,extraction with phosphate solution,followed by ammonium sulfate precipitation and hydrophobic interaction chromatography.Malanin is a glycoprotein with a molecular weight of 61875 Du and an isoelectric point of 5.5,and it was linked with two bands of A-chain and B-chain by one or more disulfide bonds.The N-terminal amino acid sequences of malanin are DYPKLTFTTSin chain-A,and DETXTDEEFN(Xwas commonly C) in chain-B.The N-terminal sequences of A-chain and B-chain had been blasted by NCBI-BLASTPand other databases with the result of no significant similarity.MALDI-TOF-MS methods were applied to measure the peptide mass fingerprinting(PMF) of malanin-Aand malanin-B,but no significant protein similarity was found by the protein database of Peptldent,suggesting that malanin is a novel protein. |
| Keywords: | |
| 点击此处可从《云南大学学报(自然科学版)》浏览原始摘要信息 | |
| 点击此处可从《云南大学学报(自然科学版)》下载免费的PDF全文 | |