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| 核酸与杨梅黄素荧光体系的研究 | |
| 引用本文： | 文志刚,赵书林,吴大旺. 核酸与杨梅黄素荧光体系的研究[J]. 云南大学学报(自然科学版), 2009, 31(4): 393 |
| 作者姓名： | 文志刚 赵书林 吴大旺 |
| 作者单位： | 1. 黔南民族师范学院化学与化工系, 贵州都匀 558000; 2. 广西师范大学化学化工学院, 广西桂林 541004 |
| 摘 要： | 研究了杨梅黄素与核酸溶液的结合反应.加入核酸溶液后,杨梅黄素在436nm处的荧光强度显著地增强.在最佳实验条件下,对ctDNA和yRNA的线性范围分别为0.2～3.2μg/mL和0.18～3.2μg/mL.并讨论了干扰物质的影响,对其作用机理也进行了初步探讨. |
| 关 键 词： | 核酸 杨梅黄素 荧光光度法 |
| 收稿时间： | 2008-11-26 |
Study of the interaction of nucleic acids and myricetin by fluorometry |
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| WEN Zhi-gang,ZHAO Shu-lin,WU Da-wang. Study of the interaction of nucleic acids and myricetin by fluorometry[J]. Journal of Yunnan University(Natural Sciences), 2009, 31(4): 393 | |
| Authors: | WEN Zhi-gang ZHAO Shu-lin WU Da-wang |
| Affiliation: | 1. Department of Chemistry and Chemical Engineering, Qiannan Normal College for Nationalities, Duyun 558000, China; 2. College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin 541004, China |
| Abstract: | The binding reaction of myricetin with nucleic acids has been studied.The experiment confirmed the fluorescence enhancing of myricetin in the presence of nucleic acids with maximum emission wavelength at 436 nm.Under optimum conditions,the calibration graphs were linear over the range of 0.2—3.2 μg/mLfor ctDNAand 0.18—3.2 μg/mLfor yRNA.Effects of interference substances on the interaction was investigated.The mechanism of the interaction between myricetin and ctDNAwas also discussed. |
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