入侵植物紫茎泽兰叶斑真菌多样性及其致病性研究

 从云南、广西等12个地区的紫茎泽兰和其他8种植物叶片上共分离得到47个真菌菌株.根据菌落、菌丝以及孢子形态,结合18SrRNA基因限制性酶切片段长度多态性(RFLP)分型和序列测定分析,47个菌株共分为了19个类群,系统发育关系与Aspergillusniger,Cladosporiumcladosporioides,Sporisoriumreilianum,Colleto-trichumlupini,Alternaria sp.,Sporobolomycesroseus,Dothideomycetesp.,Phoma sp.,Filobasidiumelegans,Aureobasid-iumpullulans,Rhodotorulaslooffiae,R.glutinis,Penicilliumdecumbens,Pestalotiopsismaculans,Cryptococcusaureus,Bimurianovae-zelandiae,Xylariaceae sp.类似.这些菌株中仅有5个对紫茎泽兰离体叶片表现致病性,在系统发育树上分别和Myrothecium sp.,C.lupini,Alternaria sp.,C.cladosporioides,Dothideomycete sp.聚类.有2个R.gluti-nis菌株对马铃薯和白菜有致病作用,但对紫茎泽兰没有致病力.

A study on the diversity and pathogenicity of the phyllosphere fungi on the invasive plant Eupatorium adenophorum

Forty-seven phyllosphere fungal strains were isolated from 12 populations of an invasive plant Eupatorium adenophorum and 8 other plants in Yunnan and Guangxi provinces of China.Based on the characters of their colonies,hyphas and spore morphology,as well as the analysis of 18SrRNA gene restriction fragment length polymorphism(RFLP) and DNA sequencing,the strains were divided into 19 groups,phylogenetically close to Aspergillus niger,Cladosporium cladosporioides,Sporisorium reilianum,Colletotrichum lupine,Alternaria sp.,Sporobolomyces roseus,Dothideomycete sp.,Phoma sp.,Filobasidium elegans,Aureobasidium pullulans,Rhodotorula slooffiae,R.glutinis,Penicillium decumbens,Pestalotiopsis maculans,Cryptococcus aureus,Bimuria novae-zelandiae,Xylariaceae sp..Only 5 strains,which clustered together with Myrothecium sp.,C.lupine,Alternaria sp.,C.cladosporioides and Dothideomycete sp in the phylogenetic tree.,were pathogenic to the detached leaves of E.adenophorum.Two strains from R.glutinis were nonpathogenic to E.adenophorum but pathogenic to cabbages and potatoes.