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| 聚乙烯醇及改性聚乙烯醇/明胶多孔支架的体外生物相容性研究 | |
| 引用本文： | 程琳,邹琴,邹立扣,阚栗强,李玉宝. 聚乙烯醇及改性聚乙烯醇/明胶多孔支架的体外生物相容性研究[J]. 成都大学学报(自然科学版), 2012, 31(2): 113-116,123 |
| 作者姓名： | 程琳 邹琴 邹立扣 阚栗强 李玉宝 |
| 作者单位： | 1. 四川农业大学都江堰校区微生物实验室,四川成都,611830 2. 四川大学分析测试中心纳米生物材料研究中心,四川成都,610064 3. 成都阚氏生物技术有限公司,四川成都,610041 |
| 基金项目： | “973计划”国际合作项目(中荷战略联盟项目)--仿生组织修复材料研究 |
| 摘 要： | 以吐温80为发泡剂,采用乳化发泡/冷冻—解冻法制备多孔的聚乙烯醇(PVA)和改性聚乙烯醇/明胶(PVA/Gel)支架材料,用扫描电子显微镜(SEM)对其形貌进行观察,并通过体外细胞培养对支架的生物相容性进行评价.实验结果表明,用该方法制备的多孔支架具有良好的贯通性,且PVA/Gel支架贯通性高于PVA支架;细胞结果显示,乳化发泡法制备的多孔支架对细胞的黏附、增殖和形貌无不良影响;荧光染色结果表明,PVA/Gel支架由于引入了大量氨基,使得细胞的黏附力优于PVA支架,具有更好的生物相容性. |
| 关 键 词： | 聚乙烯醇 明胶 吐温 生物相容性 多孔支架 |
In Vitro Biocompatibility of Polyvinyl Alcohol and Modified Polyvinyl Alcohol/Gelatin Porous Scaffold |
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| CHENG Lin,ZOU Qin,ZOU Likou,KAN Liqiang,LI Yubao. In Vitro Biocompatibility of Polyvinyl Alcohol and Modified Polyvinyl Alcohol/Gelatin Porous Scaffold[J]. Journal of Chengdu University (Natural Science), 2012, 31(2): 113-116,123 | |
| Authors: | CHENG Lin ZOU Qin ZOU Likou KAN Liqiang LI Yubao |
| Affiliation: | 1. Laboratory tff Microbiology, Sichuan Agricultural University, Dujiangyan 611830, China; 2.Research Center for Nano-biomaterial, Analytical and Testing Center, Sichuan University, Chengdu 610064, China; 3. Chenglu Kan-s Biotechnology Co. Ltd., Chengdu 610041, China) |
| Abstract: | Porous scaffolds of polyvinyl alcohol （PVA） and modified polyvinyl alcohol/gelatin （PVA/Gel） were prepared by emulsifier-foaming and freezing-thawing method with tween 80 as pore fomaing agent.Scanning electron microscope （SEM） was used to observe the porous structure of the scaffolds.The biocompat/bility of the scaffolds was assessed by co-cultured MG63 cells in vitro. The results indicate that the two scaffolds have open and interconnecting porous structure, and PVA/Gel scaffold is more interconnecting than PVA scaffold. Cell test results indicate that the two scaffolds have no negative influences on the adhe- sion,prohferation and morphology of cells. Fluorescence spectroscopy results reveal that PVA/Gel scaffold have better adhesion ability than PVA scaffold and exhibited better biocompatibilitv. |
| Keywords: | poly (vinyl alcohol) gelatin tween 80 biocompatibility porous scaffold |
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