| 超低温保存脱除两种马铃薯病毒 |
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| 引用本文： | 王子成,曲先,薄涛.超低温保存脱除两种马铃薯病毒[J].河南大学学报(自然科学版),2011,41(6). |
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| 作者姓名： | 王子成 曲先 薄涛 |
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| 作者单位： | 河南大学生命科学学院植物种质资源与遗传工程实验室,河南开封,475004 |
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| 基金项目： | 国家自然科学基金项目资助(30900973) |
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| 摘 要： | 采用超低温保存的方法对马铃薯病毒PVY和PVS进行了脱除研究.结果显示:经过超低温保存后,材料的存活率可达71.6%,比传统的茎尖培养脱毒材料的成活率在52%左右高;超低温保存后PVY病毒脱除率达到83%,比传统脱毒方法脱毒率(62%)和热处理脱毒率(65%)要高,且操作上也较简单;对于马铃薯PVS病毒脱除率在47%左右,而采用茎尖培养病毒的脱除率仅有17%左右.
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| 关 键 词： | 马铃薯 茎尖 超低温保存 脱毒 |
Elimination of Two Potato Virus by Cryopreservation |
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| WANG Zi-cheng,QU Xian,BO Tao.Elimination of Two Potato Virus by Cryopreservation[J].Journal of Henan University(Natural Science),2011,41(6). |
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| Authors: | WANG Zi-cheng QU Xian BO Tao |
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| Institution: | WANG Zi-cheng,QU Xian,BO Tao(Plant Germplasm and Genetic Engineering Lab,College of Life Sciences,Henan University,Henan Kaifeng 475004,China) |
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| Abstract: | In this study,cryopreservation method was used as a way for potato virus removal.The results showed that shoot-tip survival rate after cryopreservation was 71.6%,which was higher than 52%,the traditional survival rate of shoot-tips culture.In addition,the potato PVY virus removal by cryopreservation is feasible.The virus removal rate by cryopreservation was 83%,which was higher than traditional methods too.For example,shoot-tips culture of virus-free rate was 62% and heat treatment of virus-free rate was 65... |
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| Keywords: | potato shoot-tips cryopreservation elimination virus |
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