Class I and class II ribonuclease H activities in Crithidia fasciculata (Protozoa). |
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Authors: | H Vonwirth J K?ck W Büsen |
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Affiliation: | Biologisches Institut, Universit?t Tübingen, Federal Republic of Germany. |
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Abstract: | The protozoan Crithidia fasciculata contains two different ribonuclease H activities. These enzymes display similar physical and biochemical characteristics to their homologues in higher eukaryotes, for instance calf thymus class I and class II ribonuclease H. Class I ribonuclease H of lower and higher eukaryotes can be activated by Mg2(+)- and Mn2(+)-ions. However, the presence of Mn2(+)-ions is inhibitory for the Mg2(+)-dependent class II ribonuclease H activity of Crithidia fasciculata and calf thymus. The protozoan class I-homologue enzyme appears to be serologically related to the class I ribonuclease H of calf thymus. |
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