| 荷移反应分光光度法测定咖啡因 |
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| 引用本文： | 王彩霞,杨国玉,徐翠莲,郭中甲.荷移反应分光光度法测定咖啡因[J].河南科学,2009,27(12):1530-1532. |
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| 作者姓名： | 王彩霞 杨国玉 徐翠莲 郭中甲 |
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| 作者单位： | 河南农业大学,理学院,化学系,郑州,450002 |
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| 基金项目： | 河南省自然科学基金项目 |
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| 摘 要： | 研究了咖啡因和茜素红形成荷移络合物的反应．在pH7．5的水溶液中，咖啡因能与茜素红发生荷移反应，形成物质的量比为1：1的络合物．λmax=523．00nm，咖啡因在0．6—5．8mg／L的范围内符合比耳定律，线性回归方程A=0．00263＋0．1082c（c：μg／mL），相关系数R=0．9987，ε=2．1×10^4L／（mol·cm）．荷移反应分光光度法试剂易得，可直接在水溶液中进行，操作简单，灵敏度高，重现性好．用于茶叶中咖啡因含量的测定，取得满意的结果．
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| 关 键 词： | 分光光度法 荷移反应 咖啡因 茜素红 |
Spectrophotometric Determination of Caffeine with Charge Transfer Reaction |
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| Wang Caixia,Yang Guoyu,Xu Cuilian,Guo Zhongjia.Spectrophotometric Determination of Caffeine with Charge Transfer Reaction[J].Henan Science,2009,27(12):1530-1532. |
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| Authors: | Wang Caixia Yang Guoyu Xu Cuilian Guo Zhongjia |
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| Institution: | (Department of Chemistry, Henan Agriculture University, Zhengzhou 450002, China) |
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| Abstract: | The charge transfer reaction between caffeine and Sodium alizarinsulfonate in water solution of pH 7.5 was studied spectrophotometrically.The composition of complex is 1∶1.The apparent molar absorptivity is 2.1× 104 L(/mol.cm)at 523 nm.Beer’s Law was obeyed in the range of 0.6～5.8 mg/L of caffeine.The average recovery is 99.3 %～100.5 %,and the RSD is 1.5 %～2.8 %.The proposed method has been applied to the determination of caffeine in sample with satisfactory results. |
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| Keywords: | caffeine charge transfer-reaction spectrophotometry sodium alizarinsulfonate |
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