| 生物传感器用于葡萄酒中葡萄糖含量测定的研究 |
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| 引用本文： | 冯治平,吴平,罗泽友. 生物传感器用于葡萄酒中葡萄糖含量测定的研究[J]. 四川理工学院学报(自然科学版), 2003, 16(1): 56-58 |
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| 作者姓名： | 冯治平 吴平 罗泽友 |
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| 作者单位： | 四川轻化工学院生物工程系,四川,自贡,643033 |
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| 摘 要： | 将葡萄糖氧化酶(GOD)固定再生丝素蛋白在中,制得葡萄糖氧化酶传感器。该传感器在pH=7.0的磷酸缓冲溶液中,葡萄糖浓度在1.0×10-4 —2.5×10-3mol/L范围内呈良好线性关系。检测限5.0×10-5 mol/L,响应时间1分钟。该传感器用于葡萄酒中葡萄糖含量的测定,与酶-比色法测得结果一致。
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| 关 键 词： | 葡萄糖 葡萄糖氧化酶 生物传感器 酶电极 |
| 文章编号： | 1008-438X(2003)01-0056-03 |
| 修稿时间： | 2003-02-26 |
Determination of Glucose Content in Wine by Biosensor |
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| FENG Zhi-ping,WU Ping,LUO Ze-you. Determination of Glucose Content in Wine by Biosensor[J]. Journal of Sichuan University of Science & Engineering(Natural Science Editton), 2003, 16(1): 56-58 |
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| Authors: | FENG Zhi-ping WU Ping LUO Ze-you |
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| Abstract: | Glucose oxidase is immobilized in regenerated silk fibroin membrane. The biosensor of glucose has a linear response over the range 1.0×10-4～2.5×10-3mol/L in phosphate buffer(pH=7.0).The detection limit is 5.0×10-5 mol/L and the response time is one minute.It has same result in determination of glucose contents in wine with enzyme-colorimetric. |
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| Keywords: | glucose glucose oxidase biosensor enzyme electrode |
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