| 离子束介导大豆DNA的小麦低蛋白变异株系蛋白水解酶种类和活性分析 |
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| 引用本文： | 姬生栋,张现伟,路淑霞,祝红燕,陈鹏,王加传,袁召,岳春晖.离子束介导大豆DNA的小麦低蛋白变异株系蛋白水解酶种类和活性分析[J].河南师范大学学报(自然科学版),2008,36(2):114-116. |
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| 作者姓名： | 姬生栋 张现伟 路淑霞 祝红燕 陈鹏 王加传 袁召 岳春晖 |
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| 作者单位： | 河南师范大学,生命科学学院,河南,新乡,453007 |
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| 摘 要： | 为研究离子束介导大豆DNA的小麦变异株系在3叶期的蛋白水解酶的变化,采用复性电泳对筛选出的低蛋白变异株系05-3-1和05-15-1,作不同pH条件下的蛋白水解酶酶谱分析,结果显示:在酸性和中性条件下,与对照相比,变异株系的2102、24 kD两条酶带活性较强,均多检测到183 kD一条新酶带;在碱性条件下,变异株系05-15-1少检测到160 kD的酶带.
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| 关 键 词： | 离子束 小麦 复性电泳 蛋白水解酶 |
| 文章编号： | 1000-2367(2008)02-0114-03 |
| 修稿时间： | 2007年5月15日 |
Varieties and Activity of Proteinases in Low-protein Variants Lines of Wheat Transferal with Soybean DNA by Ion Beam |
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| JI Sheng-dong,ZHANG Xian-wei,LU Shu-xia,ZHU Hong-yan,CHEN Peng,WANG Jia-chuan,YUAN Zhao,YUE Chun-hui.Varieties and Activity of Proteinases in Low-protein Variants Lines of Wheat Transferal with Soybean DNA by Ion Beam[J].Journal of Henan Normal University(Natural Science),2008,36(2):114-116. |
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| Authors: | JI Sheng-dong ZHANG Xian-wei LU Shu-xia ZHU Hong-yan CHEN Peng WANG Jia-chuan YUAN Zhao YUE Chun-hui |
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| Abstract: | Proteinases of low-protein Variants Lines of Wheat Transferal with Soybean DNA by Ion Beam During Trefoil Stage are analyzed with SDS-G-PAGE under different PH in this paper.The results show: in contrast to control,183 kD,is tested in the low-protein offspring plants under pH 5.0 and pH 7.0;210 and 224 kD of variants took on more stronger than control;160 kD is not presented in variation a2 in comparison with control and variation a1 under pH 8.5. |
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| Keywords: | Ion beam mediation wheat renaturation electrophoresis proteinase |
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