人体尿液中阿昔洛韦和更昔洛韦的反相高效液相色谱法同时测定

研究简单的流动相甲醇-水体系,可同时对人体尿液中阿昔洛韦(ACV)和更昔洛韦(GCV)两种抗病毒药物的最优化分离,建立了通过UV检测药物的新方法。实验结果表明,流动相为甲醇-水(10/90,v/v),流速为0.5mL/min,柱温为室温,紫外检测波长254nm,使用Diamoncil C18柱对两种药物分离最好。两种药物的回收率在98.7%～101.4%之间,ACV和GCV的定量下限分别为0.08μg/mL和0.2μg/mL,信噪比为3时,最低检出限分别为0.02μg/mL和0.06μg/mL,日内、日间精密度(RSD)均小于4.8%,准确度(RE)在±5.6%之内。本方法可对样品进行灵敏、准确的定性及定量分析。

Simultaneous determination of acyclovir and gancyclovir in human urine with high performance liquid chromatography

A reversed phase high performance liquid chromatographic （RP-HPLC） method was estab- lished for the simultaneous determination of acyclovir （ACV） and gancyclovir （GCV） in human urine sample. The two anti-virus compounds were better separated by Diamoncil C is column using methanol - H20 （10： 90, v/v） as the mobile phase, the detection wavelength was 254nm and the flow rate was 0. 5mL/min. The average recoveries of the determination were between 98.7% and 101.4%. The quantum of detection （QOD） was 0.08μg/mL for ACV and 0.2μg/mL for GCV. The limit of detec- tion （LOD） （ signal-to-noise ration = 3 ） was 0.02μg/mL for ACV and O. 06μg/mL for GCV. The in- ter- and intra-day precision （RSD） were less than 4. 8%, and the accuracy （RE） was within -＋ 5.6% , respectively. The real samples analysis shows that this method can be used for the sensitive and accurate determination of the two drugs in human urine.